Hi All,
A very happy new year to everyone. I am beginning to purify a
novel kinase and was wondering if there's a standard protocol / method
that can be utilized to begin purification of a kinase. I am new to
the field of kinases and would appreciate any help with respect to the
pre
Hi All,
I am trying to express a kinase but unfortunately there is aggregation
happening as the protein is purified over a column. SInce I am new to the field
of kinase expression and purification, I was wondering if someone could provide
me with a couple of good references that can hit the
Hi All,
I am wondering if anyone can point me towards a literature where a Ser/Thr
phosphatase was expressed and purified to homogeneity which was functionally
active. Excuse my ignorance as I am new to this field and need some help
getting started.
Thanks,
neeraj
Hi all,
I recently came across a question about an interesting idea.
Does anyone know of an example of a protein binding to both Zn2+ and
Ca2+ at the same time? Are there any known well studied precedents at
all if any. Any help of insights would be very valuable.
thanks,
Neeraj
Dear All,
I have a slightly off topic question and trivial request the
citation for MOSLFM. The citation that we know is Leslie, A.G.W.,
(1992), Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography,
No. 26. but am unable to find the page numbers for the same. Could
someone k
