[ccp4bb] In-Silico Structural Biology School - Bari, October 2024

2024-05-13 Thread Mazzorana, Marco (DLSLtd,RAL,LSCI)
Dear CCP4bb’ers, On behalf of the organizing team and of the Italian Crystallographic Association (AIC) teaching committee, I am pleased to announce this year’s AIC School, organized in partnership with the GENe to Structure (GENS) team (website). Registrations a

Re: [ccp4bb] pTriEx plasmid for insect cells

2024-11-01 Thread Mazzorana, Marco (DLSLtd,RAL,LSCI)
Hi Mohinder, I successfully used modified pTriEx vectors from the pOPIN series from OPPF-UK (now PP-UK) to express a number of proteins with baculovirus as well as HEK293T cells. These vectors are great and allow quick, easy, and modular cloning of the same construct with different tags at the