Help please!
I'm looking for some new ideas. I have crystals that come out of a
sitting drop with a mixture of sodium cacodylate at pH 6.5, magnesium
acetate and MPD for the well solution. The MPD concentration is
sufficient to act as a cryoprotectant. Currently, I directly freeze
these crysta
Wow, thanks. I'm going to try to answer most of the questions I've
received in one message as I'm overwhelmed by the quick multitude of
responses.
As I stated earlier, I haven't collected any room temperature data,
yet. So, I don't know the unfrozen mosaicity. It is very possible
that the cry
Kumar and Joe-
I'm not sure if this helps but I always purified my oligos prior to
crystallization. Originally, I would order them with the trityl-group on
and purify then on a reverse phase HPLC column and used TFA to cleave on
column. Although towards the end of the project, I just ordered the
We have two systems running RHEL5. When you go to enter text in the fields
of ccp4i v. 6.0.1 or v5.0.1, nothing happens. We don't have the problem with
RHEL4. Any ideas as to what's improperly configured or I need to install.
Thanks,
Mary
Mary X. Fitzgerald
Postdoctoral Associate
Arnold Lab
R
