Hi Bill,
Peptide cutter does work for several cleavages.
http://www.expasy.ch/tools/peptidecutter/
regards
Manish
William Scott <[EMAIL PROTECTED]> wrote: Hi Citizens:
What programs/web sites would you recommend for prediction of enzyme
cleavage sites in a protein sequence?
Many thanks.
I agree with you Mark. Even in my case the twinning fractions
varied substantially among the different crystals grown in same
drop. Moreover, I feel the fractions may vary at different part of
the crystal too. Please correct me if i am wrong.
regards
Manish
Mark Mayer <[EMAIL PROTECTED]> wro
Hi Junhua & Bill,
I wonder about growing crystals in some kind of gel. Long back, i
received the crystals in gel, and they did diffract. Is it still in
practice ?
regards
Manish
William Scott <[EMAIL PROTECTED]> wrote: Hi Junhua:
These are ccp4 bb questions, so don't apologize (or we all wi
Dear Craig,
It seems Refmac is not reading the user supplied dictionary. I expected a line
near Makecif parameters like this.
"User supplied dictionary entries: /home/pathak/junk.cif"
regards
Manish
Craig McElroy <[EMAIL PROTECTED]> wrote: Hi all,
I am trying to refine a structure with a
Dear Vineet,
It seems, in your input PDB file the atom level for CA and CL in the last
column is C. It should be CA & CL respectively. The atom name in the
input file and Dictionary doesn't match.
all the best
Manish
Vineet Gaur <[EMAIL PROTECTED]> wrote: Hi all
i am having Ca2+ and Cl
Hello Yang,
You need dictionary for the residue 'abc' which will have all the
geometric restraints defined.
You can read the residue co-ordinates in Monomer Library Sketcher
in CCP4i and writeout the dictionary file using LIBCHECK. You
will be able to refine abc once you import monomer CIF di
