Re: [ccp4bb] Apparent twinning in P 1 21 1

se after refinement? > Any suggestions? > cheers > *** Dr. Linda Schuldt Department of Molecular Biology University of Aarhus Science Park Gustav Wieds Vej 10c DK-8000 Århus C Denmark

Re: [ccp4bb] Protein melting temperatures

> >> Just need a sense for whether my protein is considered unstable or >> >> somewhat stable. Please could you share some examples. >> >> >> >> Many thanks. >> >> Raji >> >> >> >> --- >> >> Raji Edayathuman

Re: [ccp4bb] Strange density on Serine oxygen.

Hi Vinson, along these lines: did you check the molecular weight of your protein with MS? This should help to answer if the molecular weight deviates from the expected one. Best wishes, Linda Savvas Savvides schrieb: > Hi Vinson > Beyond the possibility for another type of residue as already sug

Re: [ccp4bb] DTU vs DTT ?

Hi Emmanuel, it is hard for me to imagine that Coot has the wrong stereoisomer. So what I think might have happend is the following: You have imported the correct DTT, but when you have fitted the molecule into the map you might have distorted the sterochemistry at the C3 atom. And then it was ref

Re: [ccp4bb] Low resolution refinement

Ciências Farmacêuticas de Ribeirão Preto - USP > Laboratório de Cristalografia de Proteínas > Departamento de Física e Química > Fone: +55.16.3602.4193 > *** Dr. Linda Schuldt Department of Molecular Biology University of Aarhus Science Park Gustav Wieds Vej 10c DK-8000 Århus C Denmark