Jacob,
Some protein can form weak dimer, especially in low salt buffer. AUC can
provide a more detailed info about your protein dimerization state.
Ray
On Mar 22, 2011, at 8:23 PM, Jacob Keller
wrote:
> Dear Crystallographers,
>
> I have run my protein-peptide complex several times on a GE
Dear All,
I apologize for a non-crystallography question, but does anyone know the
sequence of the PreScission Protease? I would like to make it for use in my own
lab. It is just too expensive to purchase from GE!
Thanks
ray
to ask around!
Thanks again
ray
> - Original Message -
> From: "Lieh Yoon Low"
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: Wednesday, May 25, 2011 1:36:10 PM GMT -08:00 US/Canada Pacific
> Subject: [ccp4bb] PreScission Protease protein sequence
>
> Dear
Buz,
How big is the protein? How many metal binding sites are there? Do you know
what metal it should bind? What is your media?
I am assuming it is a zinc binding protein, some zinc binding protein will not
fold without enough zinc in the media. Perhaps you should not rule out
refolding.
ray
Flow rate 0.5 to 1 ml/min
Column volume ~120ml
Void volume ~50ml
On Jun 28, 2010, at 9:40 PM, Jiamu Du wrote:
> Hi, everyone
> Sorry for a non-ccp4 question. I am running a superose 6 PG XK 16/70 column
> from GE. But I can not find any technical information about this column, such
> as dead
I agree with Alex and Roger. Just a matter of choosing the right SEC column.
Ray
Lieh Yoon Low
> On Aug 20, 2014, at 4:56 PM, Alexander Aleshin
> wrote:
>
> The efficiency of a size exclusion column is proportional to the number of
> theoretical plates (plate number).
>
Millipore equipment is few
hundred ml, not sure if there is anything that can handle a few ml volume.
Ray
Lieh Yoon Low
> On Aug 20, 2014, at 6:38 PM, Alexander Aleshin
> wrote:
>
> I agree with Alex and Roger.
> But my mentioning of plates number for a concentrato
