[ccp4bb] Fluorometers

Dear all, Not a CCP4-related question, but there is no better informed group of people out there. We would like to buy a fluorometer equipped with a Peltier controlled sample cell. We will measure Trp/Tyr fluorescence, but other groups will be using the instrument for measuring fluorescence of DN

Re: [ccp4bb] what package provides libg2c.so.0 on Ubuntu ?

Dear Ed, This libg2c: http://dl.dropbox.com/u/3579334/libg2c.tgz works with older scalepack versions requiring it. It is a 32 bit library and should run on 64 bit and 32 bit systems. I have not tried the 699m version of HKL2000, but 699a and 699k2 segfault on my standard 64 bit Ubuntu (10.04.1)

Re: [ccp4bb] Strange spots

I think this is a poly-crystalline incommensurately modulated crystal, i.e. incommensurately modulated crystal, which fractured upon freezing, resulting in averaging of satellite spots. Fig. 3b from here: http://www.princeton.edu/~actin/documents/Proteincrystalscanbeincommensurate lymodulated.pdf

Re: [ccp4bb] iMosflm execution error

Dear Andy, We have seen this error since forever on our Ubuntu boxes (I believe starting from ubuntu ver. 8.04). This is what the latest version of imosflm outputs on startup: lbbspc2 ~ > imosflm MOSFLM_EXEC set to /usr/local/lbbs/ccp4/current/bin/ipmosflm [: 180: =: unexpected operator testing

Re: [ccp4bb] Projection of map?

> Dear CCP4ers, > > I want to calculate projections of an electron density map covering a > molecule along arbitrary directions. Please note: I don't want to > calculate the usual centric zone projections by using (h0l) zones and > alike, but really work on the map. Which program can I use for tha

Re: [ccp4bb] Butandiol as Cryoprotectant 2.

> > thanks so far for sharing your experience with Butanediol. But is it > important to use a specific enantiomer ? Are you all only using 2R3R- > Butanediol which is quite expensive ? Sigma Cat. # 18970 2,3-Butanediol puriss., mixture of racemic and meso forms, ≥99.0% (GC) (Fluka) works well

Re: [ccp4bb] Fitting high resolution structures to low resolution map

If it is a _true_ 4.5 A resolution map, you should be able to build alpha-helices and beta strands (can buccaneer do that automatically in at 4.5A?) and then use these secondary structure elements to find your known structures. Foldhunter from EMAN should be able to build helices and strands au

[ccp4bb] ARP/wARP 7.1; ccp 6.1.13 and Mac OSX 10.6 (64bit fink)

Dear All, My new Mac is affected by a bizarre software bug (or a feature), which has been reported on this board earlier. In fact, it has been more than a year since this problem was first reported, but it has not been resolved yet as far as I can tell. Namely, it is not possible to add the ARP/wA

Re: [ccp4bb] Coot cannot read mtz or pdb files

Many thanks to everybody who quickly replied to my cry for help. It is a locale definition problem as the laptop in question is set up with the French Swiss locale. Cheers, Petr

Re: [ccp4bb] Coot cannot read mtz or pdb files

Again, many thanks to all who responded. The simplest fix is to include this line in your .profile file: export LC_NUMERIC=C Cheers, Petr

Re: [ccp4bb] Calculation of angle between two helix of different subunits

Every other week this question comes up! This is Geometry 101 or beginner's geometry!!! http://www.euclideanspace.com/maths/algebra/vectors/angleBetween/index.htm I am not sure if it possible to understand _anything_ in crystallography if it is not clear how to calculate an angle between two vec

Re: [ccp4bb] phasing with se-met at low resolution

Dear Engin Ozkan, You have told us how bad your crystals are, but you did not mention how good your anomalous signal is: 1. To what resolution does your anomalous signal extend and what statistic is used for this estimate? 2. Do your dispersive and Bijvoet Pattersons look similar and what is the

Re: [ccp4bb] low UV reading on AKTA prime

Apologies for coming late with this comment, but this A280 filter clouding problem appears to be a common "feature" of the AKTA machines. The UV unit on these AKTAs is the same as on the old FPLC machines and the filters are interchangeable. The filters from the 10-20 year old FPLC machines fit

Re: [ccp4bb] AI and cis-peptides

> On Jul 13, 2023, at 8:12 AM, Randy John Read wrote: > > > External Email Warning: Do not click links or open attachments unless you > recognize the sender and expect the content. UTMB Email Phishing > Awareness > > I’m not sure about othe