Re: [ccp4bb] refmac,tlsanl vs TLS, B and anisou

be nice if these two refinement programs (my standard 2) could understand each other. I have been hesitant to use a phenix.refine output with ANSIOU information as a MR model through phaser, but I suspect that my hesitation is unfounded. Kevin P Madauss V-161a US

Re: [ccp4bb] Low resol structure

Jim is making from the 3.45A structure that is properly restrained and refined. Kevin P Madauss V-161a US Structural Biology GlaxoSmithKline Office: 919.483.3759 5 Moore Drive Cell: 919.924.6436 RTP, NC 27709

Re: [ccp4bb] Negative density around C of COO-

I would suspect radiation damage for the S density . I have not seen radiation damage of only COO carbons, but then again, I do not routinely get such good diffraction. Kevin P Madauss V-161a US Structural Biology GlaxoSmithKline Office

Re: [ccp4bb] problem of crystallization

I would start looking at the buffer conditions for the protein. One place to start is to reduce the salt. I would half, quarter, and then try no salt at all. I Kevin P Madauss V-161a US Structural Biology GlaxoSmithKline Office: 919.483.3759

Re: [ccp4bb] MR- Problem-76 % sequence identity-no solution

would cut 15-20 residues of the N and C terminus and try in phaser and Molrep again. Kevin P Madauss V-161a US Structural Biology GlaxoSmithKline Office: 919.483.3759 5 Moore Drive Cell: 919.924.6436 RTP, NC