Re: [ccp4bb] Off Topic: Web or e-tools for booking instrument time

Hi, all. Quartzy (http://www.quartzy.com/) is free social-networking web application for scientists that as a module for managing facilities and equipment. It looks pretty good, but I haven't used it personally. -Joel On Wed, Feb 16, 2011 at 5:05 AM, Klaus Fütterer wrote: > We use Google Ca

Re: [ccp4bb] Advice on Over-expressing and Purifying Metalloproteins

English et al. Recombinant and in vitro expression systems for hydrogenases: new frontiers in basic and applied studies for biological and synthetic H2 production. Dalton Trans. (2009) (45) pp. 9970-8 I've never tried to express a hydrogenase, but I've read that it's very hard. Do you have all nec

Re: [ccp4bb] Alignment software

Hi all. I'm rather surprised that Jalview has yet to be mentioned: http://www.jalview.org/ Although I've heard that ESPript can make the best looking figures, Jalview is also capable of generating very beautiful output, and I've found it reasonably intuitive and plenty powerful. I'm not fond of

Re: [ccp4bb] UV microscope

Hi there. I don't know how much it costs, but I've used a Korima PRS-1000 serval times, and it appears to be fairly good. Although the image quality isn't great and there is still a bit of a learning curve for identifying small crystals and/or crystals buried

Re: [ccp4bb] MS for verification of protein constructs

If you have a very pure protein sample, you'll want to use an ESI-ion trap for analyzing proteins of that size. It should be possible to get an exact mass (i.e. within a single Da). It's possible, but very rare, to get exact masses of proteins up to 100 kDa using ESI-ion trap instruments. If you

Re: [ccp4bb] Detection of DNA in protein complex crystals

The same goes for other DNA-binding dyes. I've seen a polymerase that binds EtBr. -Joel On Tue, Jun 23, 2009 at 1:38 PM, Kevin Jude wrote: > Note that, despite the claim otherwise in Kettenberger and Cramer, SYBR > Gold does stain at least some proteins, so be sure to run the appropriate >