Hi all,
We have been trying to solve a structure of protein-protein complexes using
3.1A data (one of the proteins is of 120kDa whereas the other is of 20kDa).
The structure of smaller protein is known (individually-100% sequence
identity) whereas the bigger protein does not share more than 10% s
Hi all,
We have collected few X-ray data sets for a protein-RNA complex to
resolutions of 3.2-3.5A. While processing the data using HKL2000, we
have obtained following distortion index consistently:
primitive cubic 19.16% 127.98 74.67 130.85 74.57 85.00 73.91
Hi all,
We have data sets for a protein-RNA complex at 3.2 A resolution. . The
data belong to the space group I4122 and contains one molecule of
Protein-RNA complex (300AA and 16nt; 66% solvent content) in the asym
unit. We have managed to get phases using Se-SAD and the present model
contains 60%
Dear all,
Sorry for an off-topic query.
I have been unable to crystallize a Se-met containing protein (8 Met
in 206 amino acids) in the native crystallization condition ( 0.1 M
Tris pH 8.5, 1.2 M K-Na-tartrate; Theoretical pI of protein is 8.4).
As expected, solubility of Se-Met containing protein
Hello,
I have been building a protein model (resolution 2.2A) which has one
small loop of 6 residues having poor density. I cannot see any side
chain in this region but I can see relatively poor main-chain density
which at least clearly indicate the loop conformation. I am trying my
best to build p
