in the literature values between 0.5-2% being used successfully.
Good luck.
Cheers,
Joao
Joao Dias, Ph.D.
Senior Scientist
Heptares Therapeutics Ltd
BioPark, Broadwater Road,
Welwyn Garden City,
Herts, AL7 3AX
UK
This document contains company confidential and/or proprietary information. It
is
ld also be of benefit.
Further information can be found on our website (www.heptares.com).
To apply for this position, please send your application to
h...@heptares.com, quoting reference number 2011/1B
Best wishes,
Joao Dias, Ph.D.
Senior Scientist
Heptares Therapeutics Lt
Before loading the Ni-NTA column you should exchange the medium
buffer (some media have histidine which will compete with your
protein) to a more suitable buffer, like your loading buffer (Buffer
A is 50mM phosphate buffer pH 7.5 and 300mM NaCl is OK).
If your His-tag is not accessible, then
Dear Kendall,
I would suggest you to run a simulated annealing omit map around the
region you are interested.
The model bias will be reduced and you will get a more clear answer.
Ciao,
Joao
Joao M. Dias
Ollmann Saphire Lab
The Scripps Research Institute
10550 North Torrey Pines Rd. IMM-2
La J
Petra,
You do not need any conversion.
It is true that the default FreeR flag used by the two programs is
the opposite (1 or 0), but I when you run CNS/Phenix or Refmac you
can say specifically which flag you want to use.
You just have to say in your input file which flag you want to use.
No
Hi Stephen,
I will add to the previously mentioned ones the MSD ssm server at EBI?
http://www.ebi.ac.uk/msd-srv/ssm/cgi-bin/ssmserver
You also have the Godzik tools if your proteins have flexible regions.
FATCAT
http://fatcat.burnham.org/
and POSA
http://fatcat.burnham.org/POSA/
Good luck,
Joao
Hi Eugenio,
It will depend on the expressing system you are using: mamalian
cells, insect cells, yeast.
For enzymatic deglycosylation I suggest you to do first do a small
scale test, using the following enzymes:
PNGase F is extremely efficient.
EndoH (you can try EndoHf which is cheaper and )
Jurgen,
From your question I assume that you are having problems with the
warming up of the crystal tray due to the microscope light.
Did you consider the use of an external cold light source like the
KL1500 LCD?
I do not feel anything warming up.
Which microscope are you using?
The LED sy
iculum vitae including
the names and contact details for two referees.
Please send your application in pdf format, to
h...@heptares.com<mailto:h...@heptares.com> quoting reference number 2014/2B.
The closing date for applications is 18th April 2014.
No agencies, please.
Thanks,
Joao
Dr J
Hi,
I am using the latest version of phenix 1.8.1-1168 for the MacOSX and I
see a similar behaviour with the B-factors.
Which version do you suggest to update to?
There might be a problem with over-refinement and one workaround might be
to limit the Rfree-R=0.05, but your Rfree will likely to incr
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