[ccp4bb] Protein-DNA complex for crystallization

Following up on Prof. Rice's suggestions about trying different ends and reading the literature, you might have a look at Crystallization of the yeast MATalpha2/MCM1/DNA ternary complex: general methods and principles for protein/DNA cocrystallization. J Mol Biol. 2000 Apr 7;297(4):947-59. PMID: 1

Re: [ccp4bb] highly soluble proteins

Try adjusting the pH close to the pI to reduce solubility. > From: Yvonne Leduc <[EMAIL PROTECTED]> > Reply-To: <[EMAIL PROTECTED]> > Date: Tue, 7 Aug 2007 10:17:18 -0600 > To: > Subject: [ccp4bb] highly soluble proteins > > Our lab is trying to crystallize a highly soluble (100+ mg/ml) protein

[ccp4bb] SOLVE/RESOLVE Version question

s nonsense. My question is whether anyone else has encountered similar issues with these particular versions of solve and resolve 2.13. I note that in case 3, resolve is dated earlier than solve. Could that be a problem? Thanks very much. J

[ccp4bb] Disordered domains in crystal strutures

Four examples of disordered domains in crystal structures: Disordered domains of calmodulin Wilson MA, Brunger AT. Domain flexibility in the 1.75 A resolution structure of Pb2+-calmodulin. Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1782-92. Epub 2003 Sep 19. PMID: 14501118 [PubMed -

[ccp4bb] Research Associate position at the University of Missouri

A Research Associate position (possibly two) is available to work on structural studies of proline catabolic enzymes. The successful candidate must have a Ph.D. and a strong background in protein crystallography. Applicants should submit curriculum vitae, summary of research experience and contact

[ccp4bb] PEG degradation/properties lit source

We had a similar experience a few years ago. In our case, the adventitious ligand was L-lactate, which happens to be a competitive inhibitor of our enzyme. We used using L-lactate dehydrogenase assays to show that several commercially available PEGs contain L-lactate. We also think that acetate

Re: [ccp4bb] Pymol: Zoom without Mouse

This works on a Mac laptop running 10.5.5. Go into system preferences for keyboard and Mouse. Select the trackpad menu. Click the boxes for "use two fingers to scroll" and "allow horizontal scrolling". Then, in pymol do this: 1. Place middle and ring finger on trackpad. 2. With the two finge

Re: [ccp4bb] packing diagrammes

The cns script neighbours.inp might be useful for this purpose. From: AntonioLeung Reply-To: AntonioLeung Date: Wed, 26 Aug 2009 21:46:26 +0800 To: Subject: Re: [ccp4bb] packing diagrammes Hi, Pymol may do this work, use"Action-->generate-->symmetry mates" -- Original