[ccp4bb] unsubscribe

-- Israel S. Fernandez PhD Ramakrishnan-Lab MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK. To unsubscribe from the CCP4BB list, click the following link: https

[ccp4bb] The stupid/naive question

crystals and I was wondering if the same crystal could grow in a cryo-condition or something closer to it. Thank you for investing some time in such a awkward question!!! -- Israel Sanchez Fernandez PhD Ramakrishnan-lab MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK

Re: [ccp4bb] Synthetic RNA for Crystallization

> > Graduate Student > > Biochemistry & Molecular Biology Division > > Department of Chemistry & Biochemistry > > University of California, Los Angeles > > mi...@chem.ucla.edu > -- Israel Sanchez Fernandez PhD Ramakrishnan-lab MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK

[ccp4bb] Dehydration treatments

particular topic? Thank you very much in advance -- Israel Sanchez Fernandez PhD Ramakrishnan-lab MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK

Re: [ccp4bb] How to express a >95KD FAD protein

. Ruiz-Dueñas, Patricia Ferreira, María J. Martínez, Angel T. Martínez * an in-vitro refolding of a fungal 566 AA FAD-containing protein with FAD added as refolding additive. We have managed to solve the structure. Hope it helps. -- PhD. Israel Sanchez Fernandez EM-lab Departamento de Ciencia

[ccp4bb] Weird expression behavior

protease similar to 3C that may explain the self-cleavage? why my ribosomes are not reaching until the end of the construct? Thank you so much for your attention, any comment and/or suggestion would be highly apreciated -- PhD. Israel Sanchez Fernandez EM-lab Departamento de Ciencia de

Re: [ccp4bb] Weird expression behavior

y issues w/ His tags (I think Helena > Berglund has a paper on this). > > Have you considered (after taking rare codons into account), expression > with no tag at all? pET3, pET11, etc. > > > > Ezra > > > Israel Sanchez wrote: > >> Hello crystallographers in general a

[ccp4bb]

try model-building with COOT, I did it and my body is now totally flat (like beta-sheets) and stiff (like alfa-helices)..--)) 2012/7/17 Obayed Ullah > http://www.acondicionamientos-sa.com/fevnzv.php?jrn=uoroea > -- Israel Sanchez Fernandez PhD Ramakrishnan-lab MRC Laboratory of Mol

[ccp4bb] On maps and doubts

prove a conformational change in a model phased with a molecular replacement solution? Thank you very much in advance. -- Israel Sanchez Fernandez PhD Ramakrishnan-lab MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK

[ccp4bb] Extreme off-topic on protein expression

Dear all, for reasons difficult to explain in a brief email, I am interested in flipping the order of domains in a poli-peptide in bacteria. The scenario would be a multi-domain protein, let's say containing 3 domains DI, DII and DIII, which are arranged N-ter to C-ter: DI-DII-DIII. The protein