[ccp4bb] yellow protein

2012-11-30 Thread Grishin, Andrey
Dear CCP4bb users, this problem was already discussed many times here and I've read these discussions before writing. I have a pale yellow protein. The spectrum contains peaks at 320 and 420. Before we proceed to ICP-OES and wavelength scans at the synchrotron, may be some of you had a similar

Re: [ccp4bb] To cryo or not to cryo...

2012-11-30 Thread Grishin, Andrey
Dear Yuri, I tried parathone oil once, and I can tell, that you need to be experienced in using it, so if you have some other crystals, you should get practice with them first. As for your crystal: shoot upon the matrix solvent first, and you will know is there are ice-rings or not . If yes, I

Re: [ccp4bb] comparison of different protein states

2017-02-02 Thread Grishin, Andrey
Hello Guillermo, because you are talking about conformational changes during the catalytic cycle, they should be very conserved along homologous enzymes. Discussing known examples of similar catalytic movements in homologous enzymes would strengthen your argument. During my PhD, I was workin