Dear CCP4bb users,
this problem was already discussed many times here and I've read these
discussions before writing. I have a pale yellow protein. The spectrum contains
peaks at 320 and 420. Before we proceed to ICP-OES and wavelength scans at the
synchrotron, may be some of you had a similar
Dear Yuri,
I tried parathone oil once, and I can tell, that you need to be experienced in
using it, so if you have some other crystals, you should get practice with them
first.
As for your crystal: shoot upon the matrix solvent first, and you will know is
there are ice-rings or not . If yes, I
Hello Guillermo,
because you are talking about conformational changes during the catalytic
cycle, they should be very conserved along homologous enzymes. Discussing known
examples of similar catalytic movements in homologous enzymes would strengthen
your argument.
During my PhD, I was workin