Missing density could point to incomplete data, how is the completeness at high
and low resolution?
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On May 19, 2011, at 11:54 AM, Jon Schuermann wrote:
> Dear Joane,
>
>Bert's recommendations are very good, but I'd like to add a little
> caution. If just part of the m
Are you doing MR at low resolution or just cuting data to compare with an EM
map?
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On Jun 1, 2011, at 7:35 PM, Hailiang Zhang wrote:
> Hi there,
>
> I have a preliminary question. For very low resolution data, say 10A or
> even lower, is the density map supposed to be more
You should check these "spherulites" on SDS-PAGE gel to make sure that these
contain your protein. Then you can start thinking about optimization.
On Wed, Aug 24, 2011 at 2:05 PM, Jan van Agthoven wrote:
> Dear all,
>
> I recently obtained some spherulites while trying to crystallize my
> protei
Hi,
1) maybe work on refining the protein structure first, so that your
residual maps improve in quality.
2) Maybe only part of the peptide is ordered? Can you assign part of the
sequence? Starting a bulky aromatic (e.g. W/Trip)?
3) do you have a way to QC your peptide? Is it still 11 residues in
