Re: [ccp4bb] protein stain, B-PER

ue for the last 3 months at least). Without the microwave, the same solution works well if you stain 10' and destain in water for about 1 hour. HTH, Federico Forneris, PhD --- Crystal and Structural Chemistry Bijvoet Center for Biomolecular Research - Utrecht University Padualaan 8 3584 C

Re: [ccp4bb] using pymol for making movie of change in protein structure

Hi, A quick and efficient option for morphing between multiple states (structure A --> structure B) defined by separate PDB structures is using UCSF chimera. The program will generate a user-defined number of intermediate pdb models that can be saved and used in Pymol. Best, Federico Forne

Re: [ccp4bb] [OT] which column to use in SLS/MALS instruments

high pH limit recommended by Wyatt). HTH Best, Federico Forneris, PhD --- Crystal and Structural Chemistry Bijvoet Center for Biomolecular Research - Utrecht University Padualaan 8 3584 CH UTRECHT - The Netherlands Tel. +31 (0) 30 253 2868 - Fax +31 (0) 30 253 3940 http

[ccp4bb] R: [ccp4bb] ccp4i unable to read mtz files

Check permissions of your TEMPORARY directories. When MTZ files are loaded, some dumping actions are done in those dirs. I encountered the same problem with SuSE 10.2 and solved changing permissions in /tmp/[users] directories. HTH regards, Federico Forneris -- Dept. of Genetics and

[ccp4bb] [Off-Topic] Ultracentrifuge: AirFuge vs. Conventional

the powers and the limits of this machine compared to a "conventional" ultracentrifuge. Obviously, a summary of all answers will be posted here on the bb. Thank you in advance, Federico Forneris -- Dept. of Genetics and Microbiology University of Pavia Via Ferrata, 1 27100 - Pavia (I

[ccp4bb] postdoctoral position available

, publications, a cover letter explaining research interests and fit for the position. Please also include a minimum of two personal references (name, email and phone number). The application should be sent via e-mail to Dr. Federico Forneris (f.forne...@uu.nl). Review of applications will begin

[ccp4bb] Postdoctoral fellowship on structural biology of eukaryotic complexes in Pavia (Italy)

) has a strong division in structural biology with two highy integrated and well-equipped laboratories led by Dr. Federico Forneris and Prof. Andrea Mattevi, as illustrated at www.unipv.it/biocry. Pavia is a lovely university town in the north of Italy with very good life quality. Interested candidates

[ccp4bb] Announcement: Cryo-EM School in Italy

et 50 EUR discount on the registration fee. Deadline for registration: 15 June 2017. Please note that, should we receive a large number of registrations, we may adopt a "first to come, first to serve policy". We look forward to welcoming you in Pavia. The scientific organi

[ccp4bb] AIC Cryo-EM international School 2017 - Deadlines approaching!

address or to aicsch...@cristallografia.org We look forward to hosting you in Pavia and discuss about Cryo-EM in September. On behalf of all school organizers, Very best wishes, F. Federico Forneris, PhD The Armenise-Harvard Laboratory of Structural Biology Dept. Biology and Bio

[ccp4bb] Cryo-EM School2017 in Pavia: Deadline Extended, but few places remaining

alf of all school organizers, Very best wishes, F. Federico Forneris, PhD The Armenise-Harvard Laboratory of Structural Biology Dept. Biology and Biotechnology University of Pavia Via Ferrata, 9 I-27100 Pavia - ITALY <http://fornerislab.unipv.it/> http://fornerislab.unipv.it

Re: [ccp4bb] help identifying unknown density

Any chances for some guadinium leftover from a refolding protocol? See second line in fig 1 here, quite similar stacking: http://www.mdpi.com/1420-3049/20/5/9214/htm Best, F. Federico Forneris, PhD The Armenise-Harvard Laboratory of Structural Biology Dept. Biology and Biotechnology

[ccp4bb] CALL FOR ABSTRACTS - Microsymposium about Infectious and Neglected Diseases at ECM34

ystems involved in *infectious and neglected diseases*. See you in Padua, Federico Forneris and Pedro Alzari [image: LOGO-UNIPV] <https://portale.unipv.it> *Federico Forneris, PhD* Vice-Rector for Research, University of Pavia <https://portale.unipv.it> President of the INF-ACT Foundati