On 12/20/2010 10:34 AM, Zhibing Lu wrote:
Hi All,
Recently I solved a structure in which some water molecules have
Bfactors at 0 and overall wilson Bfactor is 0.654 based on PHENIX
refinement. Is it possible?
Bill Lu
Hi Bill,
What resolution are you working with here? An overall Wilson B <
It sounds like list your GST construct is not binding to the column
(or very well) when the peptidase is attached. GST needs to form a dimer
to binding to the column - I suspect that your construct interferes with
dimer formation - when the peptidase is present, but when not there due
to stall
I cannot say what you have - running a gel, MS, etc of a washed crystal
could confirm what you have.
What you did not indicate is if your lack of diffraction was of frozen
or unfrozen crystals. I have seen too many cases where it is the cryo
condition killing diffraction. So if you have not tried
I cannot say what you have - running a gel, MS, etc of a washed crystal
could confirm what you have.
What you did not indicate is if your lack of diffraction was of frozen
or unfrozen crystals. I have seen too many cases where it is the cryo
condition killing diffraction. So if you have not tried
Take a look at
http://sourceforge.net/projects/pymol
Under develop - the svn tree is there...
Have not tried to compile it Last change four weeks ago
Ezra
On 9/21/2010 10:10 PM, William G. Scott wrote:
Hi Citizens:
I have an invoice for a PyMol academic 3 year subscription thro
On 9/28/2010 10:41 AM, Jeremiah Farelli wrote:
We recently had this problem in our lab.
No matter what we tried, we could not get convert2mtz (or any other program) to
work properly. We were probably doing something wrong with the fortran?
Depending on how far along you are, you can try
