Re: [ccp4bb] str solving problem

2013-06-18 Thread Eugene Valkov
with closest identity of 31 given a >>> contrast of 2, score 0.30 and wrfac 0.60. but "balbes" uses different >>> models with lesser identity, >>> >>> no matter which way I am going the rFree keep on increasing during >>> refinement in refmac, when I

Re: [ccp4bb] str solving problem

2013-06-19 Thread Eugene Valkov
d try shooting it in different > areas and 'searching' for a better spot to collect data. > > Or 'grow a better crystal'. > > F > > > > - > Francis E. Reyes PhD > 215 UCB > University of Colorad

Re: [ccp4bb] str solving problem

2013-06-24 Thread Eugene Valkov
2013 at 3:50 PM, Eleanor Dodson > wrote: > >> As others say - the Rfactors look pretty good for MR, mine usually start >> over 50% even with a better model and one hopes they then decrease.. >> But you say you took the Balbes model into phaser? and I think Balbes >>

Re: [ccp4bb] Phasing with Many Monomers/AU

2014-01-20 Thread Eugene Valkov
(small needles). Also, based a fluorescence scan, I know that mercury does >> not bind appreciably. Other than screening for a new space group, what >> options might I have for phasing this many monomers at lower resolution? Is >> there any real chance of solving the structure in

Re: [ccp4bb] Sister CCPs

2014-02-13 Thread Eugene Valkov
t; > > > Thus wet-lab questions are not off-topic (not that anyone recently > described them as such). > > Having said that, Jiscmail mailing lists are easy to set-up (providing > that you can reasonably expect that the mailing list will improve > knowledge sharing within th

Re: [ccp4bb] Sister CCPs

2014-02-13 Thread Eugene Valkov
till need to be > handled on a case-by-case basis, but it would be much more efficient for > everyone if the standard answers were collected somewhere permanent. > > -Nat > > > > On Thu, Feb 13, 2014 at 7:05 AM, Eugene Valkov wrote: > >> I absolutely agree with Juerg

Re: [ccp4bb] Continuing on the diffuse streak theme

2014-04-07 Thread Eugene Valkov
> something else given that they are along lattice lines? Opinions? > > --paul > -- Dr Eugene Valkov Room 3N049 Division of Structural Studies MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. Email: eval...@mrc-lmb.cam.ac.uk Tel: +44 (0) 1223 267358

[ccp4bb] Postdoctoral Positions in Structural RNA Biology at the NIH

2019-08-22 Thread Eugene Valkov
ound investigation. Applicants should send their CV and contact details for three references to Dr. Eugene Valkov: eugene.val...@nih.gov. Informal inquiries are welcome. More information can be found at https://ccr.cancer.gov/RNA-Biology-Laboratory/eugene-valkov. The RNA Biology Laboratory is located on

[ccp4bb] Repository of Unsolved Datasets in Macromolecular Crystallography?

2013-03-28 Thread Eugene Valkov
iology papers but can then use the data to improve software and computational tools that will benefit all of our community. With best regards, Eugene -- Dr Eugene Valkov MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. Email: eval...@mrc-lmb.cam.ac.uk Tel: +44 (0) 1223 407840

Re: [ccp4bb] RNA for crystallization

2013-05-20 Thread Eugene Valkov
e setting drops? > Aren't RNA synthesis protocols reliable enough nowadays for such short RNAs? > Thanks, > Alex > -- Dr Eugene Valkov Division of Structural Studies MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. Email: ev

Re: [ccp4bb] Interfaces

2018-03-05 Thread Eugene Valkov
Can you define "more complete"? What information do you require that PISA does not provide? This way you may get specific recommendations/suggestions. Eugene On 5 March 2018 at 14:02, Lorenzo Briganti wrote: > Good morning all, > > > I need to identify my dimer and tetramer interfaces, and po

Re: [ccp4bb] [External] Re: [ccp4bb] Structure prediction - waiting to happen

2023-04-02 Thread Eugene Valkov
, Eugene Eugene Valkov, D.Phil. Stadtman Investigator RNA Biology Laboratory Center for Cancer Research National Cancer Institute Frederick MD 21702, USA (301) 846-1823 On Sun, 2 Apr 2023 at 10:44, jacinto.ls wrote: > I am also not sure whether AlphaFold can address the impact of ions and ot

Re: [ccp4bb] [External] Re: [ccp4bb] Structure prediction - waiting to happen

2023-04-02 Thread Eugene Valkov
ructures. > That’s more damaging than helpful. > > > -- > *From:* CCP4 bulletin board on behalf of Eugene > Valkov > *Sent:* Sunday, April 2, 2023 10:10 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* Re: [ccp4bb] [External] Re: [ccp4bb] Structur