[ccp4bb] Problems with COOT and Pymol stereo

2012-02-08 Thread Elias Fernandez
Dear CCP4ers, I'd purchased a stereo monitor from Sharper Technology with a Planar SA2311W stereo 3D LCD and the Nvidia 3D vision kit. However, I'm having a problem with the dual monitor setup. Everything works fine with Pymol and Coot when using a single 3D capable monitor (Planar SA2311W) and N

[ccp4bb] still... problems with COOT and Pymol stereo

2012-02-17 Thread Elias Fernandez
Dear CCP4ers, I'd sent this e-mail out a week or so ago. I'm still struggling with the 3D kit and 2 monitors. I'd purchased a stereo monitor from Sharper Technology with a Planar SA2311W stereo 3D LCD and the Nvidia 3D vision kit. However, I'm having a problem with the dual monitor setup.

[ccp4bb] recombinant enterokinase

2012-03-12 Thread Elias Fernandez
Dear all, Would anyone know of a source for recombinant enterokinase? Best, Elias

Re: [ccp4bb] recombinant enterokinase

2012-03-12 Thread Elias Fernandez
Hi All, I misspoke - what I'm looking for is a source for an expression system for recombinant enterokinase. Elias Subject: recombinant enterokinase Dear all, Would anyone know of a source for recombinant enterokinase? Best, Elias

[ccp4bb] Assistant Professor Vacancy

2010-09-07 Thread Elias Fernandez
ASSISTANT PROFESSOR: The Department of Biochemistry and Cellular and Molecular Biology (BCMB) at the University of Tennessee, Knoxville seeks to fill a tenure-track faculty position at the assistant professor level to begin August 1, 2011 in the following area: EXPERIMENTAL PHYSICAL BIOCHEMIST

[ccp4bb] over-expression strategies

2013-09-12 Thread Elias Fernandez
Dear CCP4ers, We've been struggling with little (nearly none) expression of our protein, in both E coli and with in vitro transcription/translation methods. It appears that our mRNA has high 2' structure with a low dG (theoretically ~760kcal/mol). If this is indeed the source of our problem, are t

[ccp4bb] anti-His6 Ab

2013-03-15 Thread Elias Fernandez
Dear All, would you be able to recommend a primary anti-His6 Ab for western blotting? Thus far, the ones we've used are not very specific to the N-terminal hexahistidines and we pick up lots of background. Also, there's one (others?) from Sigma that is conjugated to HRP, but costs ~$650. Thanks! El

[ccp4bb] vacancy structural biologist/biophysicist faculty

2017-08-11 Thread Elias Fernandez
ASSISTANT/ASSOCIATE PROFESSOR POSITION IN EXPERIMENTAL BIOPHYSICS/STRUCTURAL BIOLOGY AT THE UNIVERSITY OF TENNESSEE The Department of Biochemistry & Cellular and Molecular Biology (BCMB) at the University of Tennessee, Knoxville (UT) is soliciting applications for a tenure‐track position at