I guess the crystals will be "safe but freaked out".
I think handling the crystal trays is a process which would much more
influence the crystal growth.
Do you ask because you have frequent fire drills or your colleagues have a
affinity for arson?
Sorry for the jokes, could not resist.
Cheers,
ed also in SHARP) is good for high resolution
data but is very strict in its data quality requests. What is your
resolution? Do you have problems with the output of the ARP/wARP at the
end of autoSHARP?
Good luck,
Djordje Francuski
What is the resolution of your data?
> Hi
>
> Sorry for
Your question just reminded me of this little tool that is nice to have.
Now this script does not really do what you ask but someone might need a
script for extracting basic secondary structure info from dssp format to
the pdb format. If anyone has something better, please share.
Take a look at Jam
Just wandering, is your DNA dissolved in a buffered solution or just water?
I would suggest to buffer your DNA solution prior mixing with the protein.
Otherwise I guess that your pH in the protein-DNA mix is lower then the
expected pH7.0 and maybe your protein does not like it.
Cheers,
Djordje
>
In short, yes you can. Although it is often avoided due to the limitations
that high salt samples may have, you still have a good chance to
crystallize the protein in the original high salt buffer. In my case a
small alcohol was the best precipitating agent. As with most proteins, try
it, try it, t
Crush, pippet up and down, dilute as needed (I used 1:200) and vortex.
Actually anything works... as everything - protein dependent.
For the stabilizing solution I have used the same as the reservoir
solution (20%MPD, 250 mM NaCl, 0.1 M HEPES pH 7.5) or 2% more MPD. Worked
fine and the seeds were
