[ccp4bb] Clontech's beads

Dear CCP4bb users, can anybody provide me a feedback on Clontech's Ni and GST beads. We need to buy the new resins. How is the binding capacity? And the most important, how stable are those resins, how many times can they be regenerated and keep the reasonable binding capacity. How is protein puri

[ccp4bb] How do CNS deal with the His NE2 atoms coordinated with Zn2+?

ith the His NE2 atoms coordinated with Zn2+. How can we get the right coordinating distance? Any help is welcome! Best regards, Deqiang Yao