Dear Angshu
The answer to your question requires to define precisely the term of
"stoichiometry".
*If you consider the hexamer as "the molecule B", then the expected
stoichiometric ratio is 1/1 (one molecule A should bind to 1 hexamer B).
*But if you consider the monomer of B as "the molecule"
Relevant to the discussion:
* Cell, Vol. 110, 551–561, September 6, 2002, Copyright 2002 by Cell Press
An RNA Thermosensor Controls Expression of Virulence Genes in Listeria
monocytogenes
* Bacterial RNA thermometers: molecular zippers and switches
Jens Kortmann and Franz Narberhaus
NATURE
I got a good advice about it: inject WhiteHouse disinfectant right into the cpu.
Good to repeat it a couple of times, but wear mask and gloves against possible
Cotrump-16-20 last attacks !
Hope it will be helpful.
Philippe Dumas
(#SupportFauci)
- Mail original -
De: "Jurgen Bosch"
À:
Monika
Did you try ITC experiments at different temperatures ?
Delta H may be null, or close to zero, at some temperature without implying
that there is no binding !
Philippe Dumas
De: "monika chandravanshi"
À: "CCP4BB"
Envoyé: Samedi 1 Août 2020 09:57:24
Objet: [ccp4bb] Regarding diffe
Dear Bernhard
I share your intuition: we should expect to observe different shapes of
titration curves depending on whether A or B2 is in the syringe (titration and
reverse titration).
I suppose that you want to test your hypothesis that, eventually, you get A2B2.
Independently of any kinetic
It seems to me that it is legitimate to refer to what you describe as
"twinning". That's the difference between merohedral and non-merohedral
twinning. What you describe is non-merohedral twinning.
In fact twinning has been (re)discovered by most of the biological community
after the remarkable
Bayesian reasoning: Given the observation of the complete identity of the 2
reports, what is the likelyhood that Bing & ChatGPT arrived at the same result
vs. the null hypothesis that Bernhard made a simple "cut & paste" mistake.
Philippe Dumas
De: "Bruno KLAHOLZ"
À: "CCP4BB"
Envoyé: V
