Re: [ccp4bb] Softwares for Protein-Protein docking!

Hi Ivan, Recently I used PatchDock (http://bioinfo3d.cs.tau.ac.il/PatchDock/index.html) to find interface between two proteins. Good luck, Biswajit Dr. Biswajit Pal, Scientist, Centre for Cellular and Molecular Biology, E009, Uppal Road, Hyderabad - 500 007, India. Phone : +91-40-2719-2831, Fax

Re: [ccp4bb] Na Acetate Buffer

s 24-38 by Vincent S. Stoll and John S. Blanchard If some one can not access the article for personal use, and others don't object to the copyright I will be very happy to share it. Biswajit Pal CCMB, India

[ccp4bb] Heme incorporation in expressed protein

expressed protein. We would like to get this protein in heme-bound holo-form. Any suggestion to trouble-shoot this problem would be highly appreciated. Replies can be sent to me directly and I will post a summary on a later date. Thanks in advance, Sincerely yours, Biswajit Pal CCMB, Hyderabad, India

Re: [ccp4bb] co-express two proteins in E.coli

I fully agree with Dima. We are able to co-express and purify two interacting partners using pET28 and pET21 in E. coli. Some related references are : J. Mol. Biol. (2011) 405,49–64 J. Biol. Chem. (2006) 281, 26491–26500 J Struct Biol. (2011) 175(2):159-70 Biswajit - Original Message -