Dear All,
I have a 100KD protein which elutes as a mixture of 90% monomer and 10%
dimer on HPLC, if I pool the monomer fractions and reload them onto the
column, the elution profile is still 90% monomer plus 10% dimer, and the
same is true if I do so to the dimer peak. Collectively, there seems
To dear Herman,
Thank you very much for your comments, I do include protease inhibitor in
the protein sample (I use Roche, which always works fine for me), and that's
why I am so surprised to see that it degrades madly. On the other hand,
since the protein behaves quite well on SEC --- nice symmet
