I realised afterwards that this might be an experimental example of desperately
trying to turn a negative result into a positive one, which is something that
has been discussed here recently (sorry, Dale) ;-0 ;-0
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Original Message
On 24 Apr 2021, 14:
Some ligands/ small molecules solubility is pH- dependent. (E.g. biotin, EDTA)
Try adjusting the pH (it doesn't have to be accurate, just use a pH
paper if you have very small volumes). It might help you out to
improve ligand solubility (and binding with your protein).
Also, not to ruin the party,
Dear colleagues,
The Section of Structural Biology of Membrane Proteins in the Laboratory of
Cell Biology, Center for Cancer Research, National Cancer Institute, NIH has an
opening for Postdoctoral Fellow with a recent Ph.D. in Biology and training in
cryo-EM and/or X-ray crystallography. This
Dear Gourab,
You might just want to add the ligand in pure, solid form to the edge of the
crystallization drop. This worked in our case beautifully (see Angew. Chem.
Int. Ed. 2012, 51, 3354). We had to soak it for 4 weeks.
Good luck!
Best regards
Bernhard
