Hello,
does the protein you added cysteine to (I assume you mean by mutagenesis) have
another cysteine which could make an intra molecular disulphide with it? That
would probably not show on non-reducing SDS?
Best wishes, Jon Cooper
Original Message
On 31 Oct 2020, 15:58, Sri
Hi Jon
there is no surface exposed cysteine nearby which can potentially
make disulfide bond with the modified cysteine intramolecularily.
Dhiraj
From: Jon Cooper
Sent: Sunday, November 1, 2020 7:41 AM
To: Srivastava, Dhiraj ; CCP4BB@JISCMAIL.AC.UK
S
Hey Dhiraj,
I saw this email yesterday, and I was waiting for others to respond. This
seems like there is no simple answer to your question. This would be
advantageous, if you kindly share the chromatograms of the WT proteins, and
the mutant one, both in the presence and absence of the reducing ag
