Hey Suchi,
you could firstly increase the binning to factor to 4-5. Also, it would help to
shrink the mask radius. Most definitely keep the resolution limited to 8-10 Å!
Cheers
Schara
Dr. Schara Safarian
Max Planck Institut für Biophysik
Abteilung für Molekulare Membranbiologie
Max Planck Ins
Dear all,
On Sat, May 30, 2020 at 03:40:53PM +0100, Eleanor Dodson wrote:
> My pennysworth. If you find your maps look better after the
> anisotroy correction use it, but it may be helpful to those wo want to mine
> your data if you deposit the whole sphere..
Agree (which is what e.g. we provide
Good evening,
since I've been contacted off line several times about this, I'm posting
here the protocol to combine maps with 2 mtz files of original and
truncated data.
I went through this procedure as I wanted to include the maps, and this
was the tricky part of the procedure in my hands. Th
Hi Everyone,
I want to crystallize a small organic molecule. I have very limited
experience in small molecule crystallography. I found that the Crystal
Screen HT from the Hampton research is good for both small molecule and
macromolecule crystallization. Plan to set up a sitting drop screen just
l
Hi
A small organic molecule is typically crystallized from organic solvents
(or water, if soluble) by means of at least three main techniques:
1. slow evaporation of solvent leading to supersaturation and eventual
crystallization
2. supersaturation at higher temperature followed by gradual drop i
Hello Jiyuan,
One small point to note- as Artem says, small molecule crystals are often
generated out of solvents and these same solvents often melt the standard
protein crystallisation plates, so be careful what you put into a plastic plate.
As Artem mentioned, synchrotrons are generally overk
