It depends on the accessibility of the cut site when bound to the column, but
we have a lot of luck with doing an on-column TEV cut of his-tagged proteins.
We load the protein on a cobalt column, mix in his-tagged TEV, and cut
overnight. In the morning the cut protein just flows off, the tag a
Dear all,
I’m currently looking for a postdoc to join my group at the Victor Chang
Cardiac Research Institute in Sydney, Australia. The successful candidate will
join a small growing group, and investigate the structure and function of large
protein complexes using cryo-EM. Experience in cryo-E
