Re: [ccp4bb] the purification process of protein, used for crystallization

In continuation of this thread, what is the success rate of separating TEV and MBP tag from my desired protein after TEV cleavage using Hydrophobic/Ion exchange column? I have also read TEV is less active in a high concentration of Imidazole, so dialysis before adding TEV may work better but then r

[ccp4bb] Three cryo-EM postdoc positions open in Helsinki

Dear All, Three postdoc positions are open in my group at the newly-established HiLIFE Helsinki Institute of Life Science. Come and work with us on cryo-EM (computational methods, structures of complexes, or viral membrane fusion). Prior expertise in cryo-EM is beneficial but not required. Univ