Re: [ccp4bb] Rmergicide Through Programming

I would like to support Graeme in his wish to retain Rmerge in Table 1, essentially for exactly the same reasons. I also strongly support Francis Reyes comment about the usefulness of Rmerge at low resolution, and I would add to his list that it can also, in some circumstances, be more indica

Re: [ccp4bb] long loop

Fab fragment binding towards long loop 在2017年07月04日 23:22，dongxiaofei 写道： Dear ALL, I want to make a protein crystal,but there is a long loop between domains of protein , which contains two small domains owning about 40 amino acids respectively and a loop about 70 amino acids. Loop is so lo

Re: [ccp4bb] Rmergicide Through Programming

>I do not see what harm there is reporting Rmerge, even if it is just used in >the inner shell or just used to capture a flavour of the data set overall. I >also appreciate that Rmeas converges to the same value for large multiplicity Consider a callow young grad student, David, who being beleag

Re: [ccp4bb] Rmergicide Through Programming

Graeme, Andrew Jacob is not arguing against an R-based statistic; he's pointing out that leaving out the multiplicity-weighting is prehistoric (Diederichs & Karplus published it 20 years ago!). So indeed: Rmerge, Rpim and I/sigI give different information. As you say. But no: Rmerge and

Re: [ccp4bb] long loop

I know that this might be considered heresy on a crystallography mailing list, but do you have any friendly NMRists at your institution? D Dr David C Briggs Hohenester Lab Department of Life Sciences Imperial College London UK http://about.me/david_briggs

[ccp4bb] Research Technician Position in Structural Biology in Vienna

Dear Colleagues I would really appreciate if you could share the following info regarding this available position. Best regards and thanks Kristina We are looking for a highly motivated research technician to join the collaborative project between Sascha Martens

Re: [ccp4bb] long loop

Do you have an inactive variant of the protein? If yes, use limited proteolysis in the presence of methylated histones to find out which flexible parts are really important and or protected by complex formation. Further, try to crystallize a complex of inactive demethylase and methylated histon

Re: [ccp4bb] Rmergicide Through Programming

Dear Frank, You are forcefully arguing essentially that others are wrong if we feel an existing statistic continues to be useful, and instead insist that it be outlawed so that we may not make use of it, just in case someone misinterprets it. Very well I do however express disquiet that we as

Re: [ccp4bb] Rmergicide Through Programming

Frank, you are asking me to remove features that I like, so I would feel that the challenge is for you to prove that this is harmful however: - at the minimum, I find it a useful check sum that the stats are internally consistent (though I interpret it for lots of other reasons too) - it is f

[ccp4bb] RELEASE NOTE SIMPLE 2.5

Dear All: We announce the release of a new version of our program package SIMPLE (v2.5) for single-particle cryo-EM *ab initio* 3D reconstruction (web: simplecryoem.com) New features include: * A new DDD movie pre-processing program that implements motion correction based the same principal stra