Re: [ccp4bb] Accidental crystallization of E. coli protein

2017-04-05 Thread zaigham mahmood khan
This may seem an interesting case, given that protocol fur purification, and subsequent crystallization is reproducible as well as this enzyme is a valid drug target for pathogenic strains of E. coli. Best Z On Apr 5, 2017 02:51, "Vipul Panchal" wrote: > I don't think it is going to be any scien

Re: [ccp4bb] Ambiguous crystal diffraction pattern

2017-04-05 Thread Kelvin Lau
Seen this before. Citrate crystals  -- Kelvin Lau Structural Plant Biology Laboratory Department of Botany and Plant Biology Science III University of Geneva 30 Quai E. An

[ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Cyprian Cukier
Dear All, We are about to buy a new imaging system for our laboratories and we consider UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about this equipment (eg. is it reliable, robust, no technical issues, low-maintenance etc.)? All comments will be appreciated. Than

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Hughes, Jon
you can save a fortune by just fitting a 590nm interference filter to the front of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my lab for about €1000 total 15 years ago and we've used it everyday since. cheers jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.

Re: [ccp4bb] Accidental crystallization of E. coli protein

2017-04-05 Thread Stefan Arold
Hi Mohamed, I don't recall such a case of a crystallized proteolytically cleaved contaminant (see our recent summary of contamination cases http://scripts.iucr.org/cgi-bin/paper?ei5009 , and references therein). I would certainly recommend depositing the data at the PDB - it may help others address

Re: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Keller, Jacob
Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a typo? JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, Jon Sent: Wednesday, April 05, 2017 6:43 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence you can

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Hughes, Jon
for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference filter. ccd chips are sensitive to infrared and transilluminators produce a lot of it! best j Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org] Gesendet: Mittwoch, 5. April 2017 14:16 An: Hughes, Jon ; CCP4BB@JISCMAIL

Re: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Keller, Jacob
But I think he was asking about imaging of intrinsic fluorescence of protein crystals… I like your idea about gel imaging, though. JPK From: Hughes, Jon [mailto:jon.hug...@bot3.bio.uni-giessen.de] Sent: Wednesday, April 05, 2017 8:23 AM To: Keller, Jacob ; CCP4BB@JISCMAIL.AC.UK Subject: AW: [cc

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Hughes, Jon
ah! ok, that's a different matter j Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org] Gesendet: Mittwoch, 5. April 2017 14:29 An: Hughes, Jon ; CCP4BB@JISCMAIL.AC.UK Betreff: RE: [ccp4bb] UVEX UV Fluorescence But I think he was asking about imaging of intrinsic fluorescence of protein cry

[ccp4bb] Post-doc in membrane protein structural biology, Karolinska Institute.

2017-04-05 Thread Joseph Brock
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Re: [ccp4bb] Accidental crystallization of E. coli protein

2017-04-05 Thread Debanu
I think it is worth reporting/publishing this crystallization and structure in addition to depositing it in the PDB and ContaMiner/ContaBase databases. Items you can consider in reporting it based on the following but can take a better decision if you complete the structure to deposition quality:

[ccp4bb] Structural Bioinformatics Training Workshop & Hackathon 2017 - Application of Big Data Technology and 3D Visualization

2017-04-05 Thread Peter Rose
Structural Bioinformatics Training Workshop & Hackathon 2017 - Application of Big Data Technology and 3D Visualization San Diego Supercomputer Center/University of California, San Diego, June 26 - 28, 2017 https://www.etouches.com/mmtf2017 This 3-day hands-on workshop introduces participan

Re: [ccp4bb] Accidental crystallization of E. coli protein

2017-04-05 Thread Bert Van-Den-Berg
I do think one has to consider whether there is a sufficient scientific advance to justify publication. After all, peer reviewers are already overworked. From the information given I don't think I would try to publish this but I'd certainly consider depositing in the PDB and contaminant database