This may seem an interesting case, given that protocol fur purification,
and subsequent crystallization is reproducible as well as this enzyme is a
valid drug target for pathogenic strains of E. coli.
Best
Z
On Apr 5, 2017 02:51, "Vipul Panchal" wrote:
> I don't think it is going to be any scien
Seen this before. Citrate crystals
--
Kelvin Lau
Structural Plant Biology Laboratory
Department of Botany and Plant Biology
Science III
University of Geneva
30 Quai E. An
Dear All,
We are about to buy a new imaging system for our laboratories and we
consider UVEX UV Fluorescence Imaging from MD. Can anyone provide some
comments about this equipment (eg. is it reliable, robust, no technical
issues, low-maintenance etc.)? All comments will be appreciated.
Than
you can save a fortune by just fitting a 590nm interference filter to the front
of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my
lab for about €1000 total 15 years ago and we've used it everyday since.
cheers
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.
Hi Mohamed,
I don't recall such a case of a crystallized proteolytically cleaved
contaminant (see our recent summary of contamination cases
http://scripts.iucr.org/cgi-bin/paper?ei5009 , and references therein).
I would certainly recommend depositing the data at the PDB - it may help
others address
Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a
typo?
JPK
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes,
Jon
Sent: Wednesday, April 05, 2017 6:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence
you can
for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference
filter. ccd chips are sensitive to infrared and transilluminators produce a lot
of it!
best
j
Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Gesendet: Mittwoch, 5. April 2017 14:16
An: Hughes, Jon ; CCP4BB@JISCMAIL
But I think he was asking about imaging of intrinsic fluorescence of protein
crystals…
I like your idea about gel imaging, though.
JPK
From: Hughes, Jon [mailto:jon.hug...@bot3.bio.uni-giessen.de]
Sent: Wednesday, April 05, 2017 8:23 AM
To: Keller, Jacob ; CCP4BB@JISCMAIL.AC.UK
Subject: AW: [cc
ah! ok, that's a different matter
j
Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Gesendet: Mittwoch, 5. April 2017 14:29
An: Hughes, Jon ; CCP4BB@JISCMAIL.AC.UK
Betreff: RE: [ccp4bb] UVEX UV Fluorescence
But I think he was asking about imaging of intrinsic fluorescence of protein
cry
Dear colleagues,
Please see the information below regarding an opening in our research group.
Please share with anyone who might be interested.
Thanks!
-Joseph.
A two-year post-doctoral position is available at the Department of Medical
Biochemistry and Biophysics (MBB),
I think it is worth reporting/publishing this crystallization and
structure in addition to depositing it in the PDB and
ContaMiner/ContaBase databases.
Items you can consider in reporting it based on the following but can
take a better decision if you complete the structure to deposition
quality:
Structural Bioinformatics Training Workshop & Hackathon 2017 - Application
of Big Data Technology and 3D Visualization
San Diego Supercomputer Center/University of California, San Diego, June 26
- 28, 2017
https://www.etouches.com/mmtf2017
This 3-day hands-on workshop introduces participan
I do think one has to consider whether there is a sufficient scientific advance
to justify publication. After all, peer reviewers are already overworked. From
the information given I don't think I would try to publish this but I'd
certainly consider depositing in the PDB and contaminant database
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