Hi,
A think a detailed discussion is off topic for CCP4 bb.
https://www.quantiki.org/wiki/quantum-sock-theory
Ben
On 2 Apr 2017, at 00:31, Phoebe A. Rice wrote:
At long last, a brilliant theoretical framework for observations previously
dismissed as mere discrepancies!
This being Saturday
Without discussion of the Sohnke groups of chiral hosons this theory is
wowefully incomplete. Gert's paper however opens up the exciting
proposition of the presence of chirase impurities in enzyme-based laundry
detergents.
Br
On Apr 2, 2017 9:48 AM, "benjamin bax" wrote:
Hi,
A think a detail
I have a similar issue because I have a lot of protein in the unbound fraction.
Maybe the his-tag is not well exposed... Have you tried to use Ni-NTA beads and
binding overnight? How long is your his-tag?
Best,
Chiara
Da: CCP4 bulletin board per conto di Mark
Dear All,
Applications are being sought for:
PhD scholarship at the Institute of Fundamental Sciences, Massey University,
Palmerston North, New Zealand
Project title: Solving the end-cap structure of a biological nanorod derived
from the Ff bacteriophage (f1, M13 or fd)
Academic mentors: A/Pro
Dear All,
We’re trying to express and purify a 1000 residue long protein and have run
into the problem that it is completely insoluble when expressed in E.coli and
is not expressed at all in insect cells. The usual tricks for improving
solubility in E.coli, such as addition of GST/MBP tags, opt
Hi,
Why don't you try adding GST/MBP tags first? This is a easy quick test.
We have a nice fusion (MBP) vector for e.coli expression if you want.
Grant
From: Sutapa Chakrabarti
mailto:chakr...@zedat.fu-berlin.de>>
Reply-To: Sutapa Chakrabarti
mailto:chakr...@zedat.fu-berlin.de>>
Date: Monday
