Hello Todd,
if your protein is a cytosolic protein (reductive environment; for ER,
Golgi, mitochondria things look different), the disulfide bond formation
you observe is most probably the result of the oxidative environment
(outside the cell) in combination with the crystal packing that that
Dear All, I have the scaled output and log file (from hkl2000) of
a dataset collected at synchrotron . The log file does not have the columns for
CC1/2, CC*, Rmeas and Rpim. I do not have access to the frames. Is it possible
to obtain the requisite parameters from just the scaled an