Re: [ccp4bb] non-specific disulfide bonds in crystal structures

2014-12-21 Thread mesters
Hello Todd, if your protein is a cytosolic protein (reductive environment; for ER, Golgi, mitochondria things look different), the disulfide bond formation you observe is most probably the result of the oxidative environment (outside the cell) in combination with the crystal packing that that

[ccp4bb] obtaining CC1/2 from old log file of hkl 2000

2014-12-21 Thread ansuman biswas
Dear All,  I have the scaled output and log file (from hkl2000) of a dataset collected at synchrotron . The log file does not have the columns for CC1/2, CC*, Rmeas and Rpim. I do not have access to the frames. Is it possible to obtain the requisite parameters from just the scaled an