Hi,
In continuation to the previous suggestion about using the 'Oligomer
Generator' in viperdb. To use viperdb, your pdb needs to be in the viper
format. So download any pdb (example this one :
http://viperdb.scripps.edu/cgi-bin/viper_coord.cgi?VDB=1z14) and then
superpose your monomer onto this r
Hi Hong,
In your PDB file if the BIOMT transformation matrix (under remark 350) is
provided, you can use this script to generate the biological assembly :
http://watcut.uwaterloo.ca/cgi-bin/makemultimer/
Greetings,
Abhi
On Thu, Apr 25, 2013 at 9:31 AM, sujata halder wrote:
> Hi,
>
> In continu
Just a reminder to register for this year's European Crystallography Meeting at
the University of Warwick, being held from 25th-29th August. Early bird
registration is only available until the 6th May.
In addition to the main meeting, The European Young Crystallographers satellite
meeting will
Hi all,
There's also a tool available for converting PDB files into the viper format
available at http://viperdb.scripps.edu/pdbToViper.php
Brad
---
Bradley Kearney, Research Associate
The Scripps Research Institute
Department of Integrative Structural and Computational Biology
Jack
VACANCY - SENIOR RESEARCHER IN GENE EXPRESSION FOR STRUCTURAL GLYCOBIOLOGY,
University of York, UK
Applications are invited for a Senior Researcher to play a major role in
establishing mammalian gene expression for 3-D structural and chemical
mechanistic studies in the area of eukaryotic glycobiol
Would PISA not do this? -- Eugene
On 25 Apr 2013, at 09:20, abhimanyu singh wrote:
Hi Hong,
In your PDB file if the BIOMT transformation matrix (under remark 350) is
provided, you can use this script to generate the biological assembly :
http://watcut.uwaterloo.ca/cgi-bin/makemultimer/
Greeti
Dear Hong,
As Eugene mentioned, you should be able to build the full virus assembly using
PISA.
Another option is to use the multiscale model menu of UCSF Chimera (default
setting is "Biological Assembly") to generate the complete virus assembly from
PDB BIOMT records.
If this is a new struct
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Careina,
One thing to try if other ideas don't work or are too difficult, is
covalently (therefore unambiguously) labelling a little of your protein
with a fluorescent dye. If you add 20 nL of this to the drop *after the
crystals have grown*, protein crystals will light up, but salt crystals
will
Dear CCP4 colleagues.
I'm just finishing up a refinement, but am left with one little curio that I
just can't seem to solve.
One aspartic acid residue is associated with some extra, unexplained electron
density.
--> please see: http://i.imgur.com/vCYOqam.png
Where, the Fo-Fc map is contou
Call EBI, consider some kind of chemical modification.
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: mbfro...@post.tau.ac.il
Tel: ++972
Dear colleagues,
i hope every one doing well. i am trying to remove the 10x his tag , as i tried
to crystallize with the tag but i didn^t obtain crystals unless some salt
crystals , very small crystals and some precipitant. i want to use factor 10a
protease but i didn^t tried it before. this is
Hello Tony
is that Asp-Gly? If so, it could be prone to succinimide formation. Check out
this paper:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2323960/
and references therein!
Good luck
Jon.Cooper
--- On Thu, 25/4/13, Antony Oliver wrote:
From: Antony Oliver
Subject: [ccp4bb] Curious electr
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