Hi all.
I have small and fragile membrane protein crystals with size 40 micron. They
like to break when I try take them out with regular loops for freezing. Is
there any special tricks to handle them for data collection?
Thank you.
Theresa
Dear Ron,
Quite so, and who cannot laugh at the Yes Minister perfect hospital ward
operating theatre sketch ( Thankyou James W).
Anyway:-
Let's not get too hung up on one detail of your point 3. Your various points,
including point 3, added several missing elements in this CCp4bb thread.
Over
Something the developers might be interested in:
The Refmac_5.6.0117 32-bit windows binaries run native on a win64 3-4x
slower than
those from the linux distribution run
**in a RHEL6.2-64 VMware virtual machine hosted the same windows7/64
system.**
VM/RHEL:
Refmac_5.6.0117: End of Ref
I don't know the state of current software, because I haven't tried
recently, but when I set up my student crystallography workstations a
few years back I noticed many packages (e.g. EPMR, Phaser) that had
potentially long run times (where it is really noticeable) would run on
the identical har
On Sat, Apr 7, 2012 at 9:50 AM, Roger Rowlett wrote:
> I don't know the state of current software, because I haven't tried
> recently, but when I set up my student crystallography workstations a few
> years back I noticed many packages (e.g. EPMR, Phaser) that had potentially
> long run times (whe
I doubt many people completely fail to archive data but maintaining data
archives can be a pain so I'm not sure what the useful age of the average
archive is. Do people who archived to tape keep their tapes in a format that
can be read by modern tape drives? Do people who archived data to a ha
Just came across it and thought it's quite relevant with regard to the
latest furor over fraud in crystallography:
http://www.nature.com/nature/journal/v483/n7391/pdf/483531a.pdf
"53 papers were deemed 'landmark' studies ... scientific findings were
confirmed in only 6 (11%) cases".
Mean num
along those lines one should also look at this NRDD paper:
http://www.nature.com/nrd/journal/v11/n3/full/nrd3681.html
Rather long but has lots of very useful citations.
If you ask industry people it's a know problem that 50% of their own
experiments can't be reproduced but it's even worse if you
Have you tried the MiTeGen Micromesh? They're my favorite for handling
tiny crystals.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
h...@hawaii.edu
On Sat, Apr 7, 2012 at 1:00 PM, CCP4BB automatic digest system
wrote:
>
> Hi all.
>
> I have small
Thank you for all the on and off-board replies.
The suggestion is to use meshed loops from Molecular Dimensions or Mitegen. I
presume both works well to collect data.
Theresa
Hi Bernhard,
> Maybe the paranoia-checkers in windows slow everything down
> although I did not see any resources overwhelmed...
I wonder whether the windoze refmac binaries can be used through wine in a
GNU/Linux environment. If yes, then you could possibly differentiate
between the operating-
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