Hi all,
I have a strange result using Phenix's AutoSol to look for xenon sites in
lysozyme.
For a few months now I have been trying to produce xenon derivatives of
lysozyme using pressures in the range of 50-350psi and time ranges between
5-60min trying to find the "sweet spot" so that this
When you crystallized your lysozyme, did you have any other anomalous
scattering atoms in the conditions? I am thinking of chloride ions in
particular, but there could be many others. How did you distinguish between
sites of xenon and sites of other kinds of atoms in your analysis?
Did you do
Dear Brennan,
Since you accidentally sent your request to the CCP4bb rather than the
Phenix email list, I suggest that you try SHELXC/D/E, e.g. using Thomas
Schneider's HKL2MAP, to see if it gets the same answers. These programs
have solved lysozyme hundreds of times. HKL2MAP will also automat
Hi all.
Does anyone have experience in using dual polarization interferometry (DPI) to
study conformation change of protein when binding ligand? How do this compare
to SAXS? I exclude NMR because the protein is large 90 kDa and no crystal
structure is available.
Thank you.
Theresa
Dear Colleague
This is a reminder that for the
Seventh International Workshop on X-ray Radiation Damage to Biological
Crystalline Samples, Diamond Light Source 14-16th March 2012 the closing date
for submission of abstracts to be considered for Oral presentations will be
Monday 6th February 201
Hello Everyone,
Many many thanks to all the folks who responded to my question with very
good suggestions.
Here's a very quick and dirty summary of the various tubes and rotors that
people use without any issues:
(1) 50ml Nalgene tubes for an SS-34 rotor
(2) Shape-matched new Fiberlite rotors
(3)
Clearly, it's better to use shape-matched rotors (I sort of assumed
that you do that already!); however the BD/Falcon polyethylene tubes
(conical ends) will actually change shape (flow) when placed into the
round-ended rotors, if the speed is high enough -- and most of the
time the tubes survive th
P.P.S. it also matters a LOT how you fill the tubes. Leaving too much
air gap on the top is actually very likely to cause crushing of the
plastic.
On Thu, Feb 2, 2012 at 6:18 PM, Artem Evdokimov
wrote:
> Clearly, it's better to use shape-matched rotors (I sort of assumed
> that you do that alread
Dear members,
Apologize for this off-topic question. I am looking for a protein sequence
alignment tool which is capable to generate a particular output file
similar to the attached format (please see the attached picture). I have
been looking at some popular programs but none of them can show the
On Thursday, 02 February 2012, you wrote:
> Dear members,
>
> Apologize for this off-topic question. I am looking for a protein sequence
> alignment tool which is capable to generate a particular output file
> similar to the attached format (please see the attached picture). I have
> been looking
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