Hi Peter
You are right: the location of the prism experiment is most likely the
study at Woolsthorpe, e.g. see
http://www.isaacnewton.org.uk/texts/OfColours7 . Newton was admitted
to Trinity College in 1661 as a 'sizar' (a paid part-time student
employed by the College) but was forced to return t
Rashmi,
There are other membrane materials which might bind less. We have also found
that there seems to be binding sometimes to the plastic itself. So a
different manufacturer can help.
Also, always use same centricon for same protein. Sometimes you take a big
hit on a centricon's first
Another option I didn't mention is presoak your centricons in 30% glycerol over
night prior to usage.
Jürgen
On Jan 28, 2012, at 12:47 PM, Bosch, Juergen wrote:
Are you close to the theoretical isoelectric point of your protein ? Change pH
of buffer
Jürgen
..
Jürgen Bosch
J
Jacob, here's my (personal) take on this:
The data quality metrics that everyone uses clearly fall into 2
classes: 'consistency' metrics, i.e. Rmerge/meas/pim and CC(1/2) which
measure how well redundant observations agree, and signal/noise ratio
metrics, i.e. mean(I/sigma) and completeness, which
For the history buffs and crystallographers needing some R&R and chill-out,
an interesting historic fiction read about the era of Newton and Leibnitz
and the foundation of the Royal Society is the Baroque cycle by Neil
Stevenson.
http://en.wikipedia.org/wiki/The_Baroque_Cycle
Cryptonomicon, althoug
Rashmi,
I had a similar problem when I used an amicon to concentrate my protein, your
buffer composition indicates me that a lot of salt concentration and glicerol
makes your protein soluble, I had a similar buffer with a lot of saltt and 15%
glicerol, and I simply reason that my protein it is
Just one thing to add to that very detailed response from Ian.
We've tended to use a slightly different approach to determining a sensible
resolution cutoff, where we judge whether there's useful information in the
highest resolution data by whether it agrees with calculated structure factors
c
Call for access to Synchrotron Beamline Facilities 2012
* DEADLINE 31/01/2012 24:00 CET *
EMBL Hamburg, Germany
We announce a call for synchrotron beam time applications in biological
small-angle scattering (SAXS) and macromolecular crystal
Hi all,
In the context of the above going discussion can anybody post links for a
few relevant articles?
Thanks in advance,
ARKO
On Mon, Jan 30, 2012 at 3:05 AM, Randy Read wrote:
> Just one thing to add to that very detailed response from Ian.
>
> We've tended to use a slightly different app
