It is a bit of a mystery to me why two structures of supposedly similar
resolution with equally acceptable maps can give very different r
factors - one sticks in the low 20s and another gives a smug 17% ..
I guess one could go back and analyse the model against the data and time..
Eleanor
On
Dear crystallographers
I have a protein of 90 kDa forming dimers. Crystals formed with microbatch and
vapor diffusion method in 24 hours but no diffraction at home source. Dissolved
crystals was confirmed to be the protein with mass spec.
Any suggestions to improve diffraction would be welcome.
Might I suggest consulting the CCP4 user community wiki on the topic:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality
Good luck,
Katherine
On Thu, Jan 26, 2012 at 9:33 AM, Theresa H. Hsu wrote:
> Dear crystallographers
>
> I have a protein of 90 kDa formi
But first of all: try to add a synchrotron to the crystals
Poul
On 26/01/2012, at 16.48, Katherine Sippel wrote:
> Might I suggest consulting the CCP4 user community wiki on the topic:
>
> http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality
>
> Good luck,
>
>
I think that all these points are interesting and valid.
On Jan 25, 2012, at 10:37, Chris Morris wrote:
Tassos reports:
1. None of the twenty test-users was satisfied with any of the two
solutions - and each was annoyed for a different reason.
This suggests that the choice of ELN is not the
And ambient if you were using cryo ...
Edward Snell Ph.D.
Assistant Prof. Department of Structural Biology, SUNY Buffalo,
Senior Scientist, Hauptman-Woodward Medical Research Institute
700 Ellicott Street, Buffalo, NY 14203-1102
Phone: (716) 898 8631 Fax: (716) 898 8660
Skype: edd
Ditto to Poul's advice.
I've had many many many cases where crystals diffract poorly (or not at all) on
home sources only to show excellent diffraction at a synchrotron. (Whether or
not a home source is properly calibrated is probably the biggest issue, but
that's for another discussion).
It depends a lot on which home source and which synchrotron, there are
enormous differences. Goettingen is uniquely well placed because we can
reach four synchrotrons in a few (3-7) hours by high speed train and in
theory at least five more with a longer train journey, trains are very
convenien
As other have/surely will suggest, by all means
try RT collection to establish if your crystals really diffract
OK, or if your cryo conditions are killing them. We have lots of
experience in our lab getting "beamstop" diffraction with certain
samples when subjecte
Theresa
You should also try microseeding into *random screens *by making a seed
stock with the crystals that you have, to use with both microbatch and
vapor diffusion experiments. You will often pick up new and better
conditions and you're more likely to get well-formed crystals right out of
the
As the proud owner of a carefully organized, highly annotated VMS backup tape
(reel-to-reel, of course), my main concern is that paper is the only format
that we'll be able to count on reading a decade (or more) from now.
=
Phoebe A. Rice
Dept. of Biochemistry
For crystallization:
Your xtal may come out a little bit fast. If the condition contain
alcohol, such as IPA, you may have to modify it.
If you let people know the condition, it may be more helpful.
Also, please check the purity of your protein.
Kevin
On Thu, Jan 26, 2012 at 7:33 AM, Theresa
Maybe, you can adjust the ion strength of your condition.
On Thu, Jan 26, 2012 at 8:32 AM, Kevin Jin wrote:
> For crystallization:
>
> Your xtal may come out a little bit fast. If the condition contain
> alcohol, such as IPA, you may have to modify it.
>
> If you let people know the condition
Hi there
we are trying to install Coot onto one of our old SGIs and so we installed Coot
0.0.33 (IRIX). However when starting Coot, such as in the Coot (install)
directory, we get an error message stating thatlib "libgcc_s.so is required
but can not be found. We have the sgi freeware gcc_lib
Unless you have written on the paper using cursive script. Many schools
in the US have stopped teaching longhand reading/writing so in a generation
or two many paper records will be undecipherable to all but historians. My
wife has some handwritten letters from ancestors written in German arou
Can't you get a plug-in for that?
JPK
On Thu, Jan 26, 2012 at 11:35 AM, Dale Tronrud
wrote:
> Unless you have written on the paper using cursive script. Many schools
> in the US have stopped teaching longhand reading/writing so in a generation
> or two many paper records will be undecipherabl
Merging in radiation damaged data can indeed raise R/Rfree because the
structure factors no longer correspond to the native structure. Rather,
they are an intensity-average of the native and damaged structures, and
that can be hard to fit to a coordinate model! How much damage is too
much?
*POST-DOCTORAL POSITION*
Three post-doctoral positions are available to join a multi-disciplinary
research group investigating the regulation of biological motors activity
from the cellular to the molecular level. The group is formed by the
laboratories of Gary Gerfen, Ao Ma, David Sharp and Her
Dear ccp4 win developers: I would like to compile Ian's EDSTAT program using
my windows7/64 ifort compiler. I have a few questions re library linking and
win-compliable source distribution - wonder who I may kindly harass
off-board.
Best regards, BR
---
Dear Gina:
I think Coot 0.0.33 originated sometime early in the Nixon administration, and
I finally parted with my SGIs a few years ago, so am not in a good position to
advise. I seem vaguely to remember some non-canonical naming of the files.
What happens if you make a symbolic link from the
Dear All,
I was wondering if there is any IOS5 based app to display protein models,
which are not in public database, on Iphone. There is an app called
Molecules for displaying models but that utilizes coordinates from RCSB and
pubchem.
Thanks,
Shiva
On 01/27/2012 04:00 PM, Shiva Bhowmik wrote:
Dear All,
I was wondering if there is any IOS5 based app to display protein
models, which are not in public database, on Iphone.
Better than this: Jolecule from Dr. Bosco Ho.
"Jolecule works in HTML5 browsers such as Chrome and Safari, mostly in
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