It helps to keep the same water naming convention in all the complexes.
distang is a slow tool but it will list all contacts to a given set of
atomic radii. I use that output a lot to check on interesting contacts..
But in the end it boils down to thoughtful book-keeping..
Eleanor
On 06/14/
It looks like a rhombehedral data set with small deviations from the
exact H3 symmetry to give the weak spots.
The standard H3 setting has origins at (0,0,0) (1/3, 2/3, 2/3) and
(2/3,1/3,1/3) so to get your translation vector to match the
conventional H3 one, you will have to reindex the P321
Brian,
We use a PlateLoc Thermal Microplate sealer from Agilent. Works very
well, pricey though ...
Cheers,
Carsten
> -Original Message-
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Brian Mark
> Sent: Tuesday, June 14, 2011 5:19 PM
> To: CCP4BB@JISCM
You might also want to try:
http://www.ncbi.nlm.nih.gov/pubmed?term=12499536
Cheers,
Stephen
On 15 June 2011 02:09, Robbie Joosten wrote:
> Hi Wolfram,
>
> This was an early study on the subject:
> http://www.ncbi.nlm.nih.gov/pubmed/8594192
> The software is still accessible via the STAN serve
Dear all,
Does anyone have suggestions for 6 Å resolution phasing with large
number (40-50) Se sites (SAD so far)??
Thanks a bunch,
Tommi
Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. B
Hi Tommi,
Give it a try?
You give very little information, e.g.
- the data quality,
- possible radiation damage,
- isomorphism with a native data set, and
- what you have tried so far.
If you have a native data set with an isomorphous unit cell, try SIRAS instead
of SAD.
Tim
On Wed, Jun 15,
Dear friends,
A zinc atom is located in the active center of my protein.
I want to estimate the errors of bond-lengths between the Zinc and
coordinated residues in the crystal structure.
Who knows how to estimate that errors?
Thank you very much!
If you have atomic resolution data you could use shelxl to invert
the least-squares matrix and calculate standard uncertainties for all
the bond lengths and angles.
Dale Tronrud
On 06/15/11 07:57, Tian-Min Fu wrote:
> Dear friends,
>
>
>
> A zinc atom is located in the active center o
Since Frank mentioned cryotongs, I wanted to point to a video of their use
on youtube:
http://www.youtube.com/watch?v=ikiF2qpCRKs
Jim
On Wed, 8 Jun 2011, Frank von Delft wrote:
Hi Zhijie --
With all manipulations on the goniometer, I strongly advise what I learnt
from Elspeth Garman: pra
[ Postdoctoral Research Fellow - Genome Damage and Stability Centre, University
of Sussex - Ref 292 ]
An MRC-funded post is available in the laboratory of Professor Laurence Pearl
and Dr Antony Oliver to study the structure and function of the Smc5/6 complex.
This project forms part of a coord
Based on the dents in my own forehead from banging it on similar brick walls,
it would probably be more efficient to get a heavier atom with fewer binding
sites, and work your way in from there. If your data set really only extends
to 6A, even when you do find all those Se atoms their phasing p
Thank you everyone for your replies. The Nayal & Di Cera (1996)
paper may be what I had in mind. I was looking for some estimate of
how often water atoms are placed in protein models where they do not
belong, and I expected a relatively high percentage. Simply
extrapolating from the 0.01% water <->
While we are on the subject of tools for crystallography, I wanted to
bring to your attention a nice tool that we use at the SGC to assist
in the harvesting of crystals into Rigaku style pucks. The Quick Puck
Loader is available at 1degreebio.org.
http://www.youtube.com/watch?v=5okdJfi3kFI
John
Hi Nigel,
I need to make a cif file for a gold ion, Au1+, no other atoms. It is
covalently attached to sulfur of cysteine
in two conformations. So, how do I label Au+1 with A and B
conformations. How to make a cif for this.
Maia
Hi,
Thanks for all the comments, i was more wondering what the state of
the art might
be here, we have done similar thing at 4 Å (with 3 Å data though) 10
years or so back
with SnB. Maybe we need to get a heavier atom derivative indeed the
break the phases,
but i'll keep banging for now..
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