[ccp4bb] Detergents

Hi, I had set up crystallization with a bicine as buffer and peg 400 as precipitant. I used the detergent DDAO/LDAO as an additive to the crystallization drop (one of the hampton additive screen condition, it says 5% on the vial) I have a clear drop and in the centre there is a shiny precipitate (

Re: [ccp4bb] mtz2various command line R-free label?

I dont know - it works for me.. That is under Fedora x Eleanor mtz2various HKLIN "./ins_pig_zn_T2_hex-unique_refmac1.mtz" HKLOUT "./ins_pig_zn_T2_hex-unique_refmac1.hkl" OUTPUT CNS labin FP=FP SIGFP=SDFP PHIB=PHIC FREE=FreeR_flag end On 04/27/2011 07:26 PM, Kelly Daughtry wrote: Yes,

Re: [ccp4bb] Map correlation coefficient

I guess one way would be to seperate coordinates.. But doesnt overlapmap do that by default? Eleanor On 04/21/2011 10:25 PM, Maher Alayyoubi wrote: Hi Everybody, I posted a question earlier on the bulletin regarding how to calculate the map correlation coefficient using Overlapamp or any othe

Re: [ccp4bb] Detergents

Rashmi, Yes, you should use the needle for seeding. The first thing to do is to seed your control drop to make sure that the needles are not bicine, as this buffer can give needles at certain pH at high PEG400 concentrations. Next (or at the same time) also seed other protein drops that you

[ccp4bb] database search within the PDB

Dear all, I would like to search the PDB for protein structures with the conditions that the biological unit consists of a homo-tetramer and that the monomers are related by a fourfold rotation, i.e. point group 4. The rcsb.org server allows to specify the number of chains in the biological unit,

[ccp4bb] Staff scientist for the Core Facility ,Structural Biology (protein expression) in Vienna

The Campus Science Support Facilities (CSF) are looking for a   Staff scientist for the Core Facility Structural Biology (protein _expression_)   Campus Science Support Facilities (CSF) were

[ccp4bb] Staff scientist for the Core Facility ,Structural Biology (macromolecular crystallography) in Vienna

The Campus Science Support Facilities (CSF) are looking for a   Staff scientist for the Core Facility Structural Biology (macromolecular crystallography)   Campus Science Support Facilities (CSF

Re: [ccp4bb] Using chaperones to boost expression in E. coli

Hi, I wanted to thank everyone for their responses to my inquiry. It's good to see that there have been positive examples, and also that one should not be discouraged from trying by initial negative results. Thanks again, and have a nice weekend, Michael __

[ccp4bb] Job opportunity

Hi, Here is a position in a company in the US for a protein crystallographer. JL -- Jean-Luc Ferrer |---| |Institut de Biologie Structurale J.P. Ebel CEA/CNRS/UJF| |41 rue Jules Horowitz - 38027 Grenoble cedex 1 - FRANCE| |tel.: +33 (

[ccp4bb] Phenix version 1.7.1 released

The Phenix developers are pleased to announce that version 1.7.1 of Phenix is now available. Binary installers for Linux, and Mac OSX platforms are available at the download site: http://phenix-online.org/download/ Some of the new features in this version are: Graphical Interface -

Re: [ccp4bb] Error in LABIN

Here is more information on my problem: I installed ccp4i in /usr/local as root using the automated install.sh script. I then sourced the ccp4.setup and ccp4-others.setup for the proper shell, making sure the environment variables were pointing in the right direction. Now, if I run ccp4i as a