Hi Zhiyi,
There are two ways you can go about this (RT data collection). Either mount the
crystals in capillaries (not all beam lines at synchrotrons have sufficient
space on the goniometer setup to allow data collection on capillary-mounted
crystals) or use a Humidifier (the latter is briefly
Francois, the possible non-equivalent alternate origins for F432 are:
1 0. 0. 0.
2 0.2500 0.2500 0.2500
3 0.5000 0.5000 0.5000
4 0.7500 0.7500 0.7500
plus of course the symmetry-equivalent origins generated from these 4 by
the space-group centring (F) t
Zhiyi,
You can use a thin cap over your cryo loop, just put a drop of mother
liquor in the top, place over the loop and make it airtight at the base.
Not sure who sells these things though, I guess you can make it from a
capillary too. Then remove the cryo stream or put it at a temp above
fr
John R Helliwell wrote:
Dear James,
I enjoyed your simulations.
Thank you!
Re your conclusion:-
"However, if the disorder is correlated across the entire mosaic
domain, then the "diffuse scatter" intensity pattern migrates from in
between the spots to influencing the spots themselves!
Hi Fred and Zhiyi,
the EMBL and ESRF have also developed a dehydration device that can
also be used for room temperature data collection, see the website here:
http://www.esrf.fr/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
http://scripts.iucr.org/cgi-bin/paper?S090744490
James
> Two-headed side chains or TLS will cause
> potentially interpretable DS features
I think the point here (probably the one you are making) is that if
crystallographers produce a pseudo rigid body motion (or static
disorder) model described by TLS parameters then it would make specific
predi
Ian Tickle wrote:
Francois, the possible non-equivalent alternate origins for F432 are:
1 0. 0. 0.
2 0.2500 0.2500 0.2500
3 0.5000 0.5000 0.5000
4 0.7500 0.7500 0.7500
It correspond to what is found at the end of James Holton's
origins.com script
htt
Francois,
> However, from what you say, I understand that only 2x3 possible
> origins with each coordinate being 0 or .5 should be accepted by
> reforigin.
No, 4x4 (i.e. the original 4 non-equivalent + 3x4 equivalent to these),
specifically:
1 0. 0. 0.
2 0.2500 0.2500
MiTeGen (www.mitegen.com) sells a RT device - plastic capillary to put
over loop... I do not know how well it would work for a long data
collection - but people here have used it to evaluate their crystals
Ezra
On 1/27/2010 3:58 AM, Flip Hoedemaeker wrote:
Zhiyi,
You can use a thin cap
MiTeGen have instructions on their website for using their system. In
practice I usually put a little silicon grease at the base of the
capillary as sometimes the capillaries fall off plus you need reasonable
steady hand eye coordination to put the capillary over the loop...I have
found MiTeGen R
Dear Al,
Refmac by default divides reflexions in 20 shells ("bins") - this can
mean a few of them do not contain a thin Rfree shell.
When this happens I add "bins 10" to the refmac com-file, or, in
CCP4i, instead of "run", do "run and view com file" and add "bins 10"
to the file before conti
in my opinion one should always first check RT diffraction and we use
the Mitegen plastic cap over the loop. We find the solutions slowly
dry out (6-18 hours), which can be enough to get a useful RT dataset
on the rotating anode.
If you like, the crystal can also be recovered after RT diffrac
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Francois Berenger wrote:
It correspond to what is found at the end of James Holton's
origins.com script
http://bl831.als.lbl.gov/~jamesh/pickup/origins.com
so I guess it should be correct.
Uhhh...
He also says how he found them:
# TABLE OF ALLOWED ORIGIN SHIFTS
These origin shifts were determ
... and I was assuming that since it was clear that there are omissions
for F222 and F23 on the CCP4 'alternate origins' page, that James' page
was 100% correct! But I think we have both made the same mistake of
assuming that F222, F23 and F432 all behave identically as far as origin
shifts are co
Correction: my apologies, I see now that the table of Cheshire groups
that I referred to originally came from: Hirshfeld (1968), Acta Cryst.
A24, 301-311. I got it from Jorge: it's still good to know that it has
Jorge's seal of approval!
Cheers
-- Ian
> -Original Message-
> From: owner
Hi,
If you go to
http://cci.lbl.gov/cctbx/explore_symmetry.html
fill out any space group (F432), and search for:
Additional generators of Euclidean normalizer
to get
Number of structure-seminvariant vectors and moduli: 1
VectorModulus
(1, 0, 0) 2
The seminvariants for F432 a
Two cents are added here.
First, try P2 as somethimes systematic absence along b axis is misleading
due to weak diffraction or pseduo translation.
Second, try P1.
Good luck,
Donghui
On Tue, Jan 26, 2010 at 1:50 AM, Michele Lunelli wrote:
> Dear all,
>
> I am trying to solve a structure at 2.
Hi Zhiyi,
I think the easiest way is to mount your crystal in an
air-impermeable, viscous oil like Paratone in a loop. Of course, your
crystals may not tolerate oil.
ho
Colin,
Your point:
"I think the point here (probably the one you are making) is that if
crystallographers produce a pseudo rigid body motion (or static
disorder) model described by TLS parameters then it would make specific
predictions of diffuse scatter. These predictions could be used to test
th
On Wednesday 27 January 2010, John Badger wrote:
> Colin,
>
> Your point:
> "I think the point here (probably the one you are making) is that if
> crystallographers produce a pseudo rigid body motion (or static
> disorder) model described by TLS parameters then it would make specific
> predictions
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