To avoid the creation of a cumbersome new unit everyone will need to
keep track of, can we just come up with a prefix that means 0.013 of
something? Perhaps we could give it the symbol "b" and then we could
say "the B-factor is "20 bA^2".*
James
*Seemed like 76.92 b humor units when I wrot
Maybe... Depending on the energetics - it could stall the translation -
or prevent initiation if it is at the start of the ORF. If it is in the
middle - you might be ok... Usually the gene synthesis company can
recommend alternate sequences that do not run into this problem or rare
codon issues
Dear Sangeetha,
Can I assume that you detected the hairpin by computational means? In
general a single hairpin may not necessarily doom your experiment or reduce
expression - however you could reduce the hairpin stability via silent codon
changes just to be on the safe side. Keep in mind that i
This question by the "Mad Scientist" (here after the MS) has provoked
me to give the topic a lot of thought. I think I can provide some
direction towards the solution, but I'm not adept enough with "The
Optical Principles of the Diffraction of X-rays" (Which people on this
BB should refer to s