Jakob,
That Compacdt disc appearance is very familiar, those were right next to
the hexagonal ones in my experiments. And all were oily when touched. It is
very likely that my "detergent" crystals were in reality detergent-protein
complexes with less than crystalline order. I was doing experiments
On 8/5/09 5:30 AM, "Jose Antonio Cuesta Seijo" wrote:
> Jakob,
>
> That Compacdt disc appearance is very familiar, those were right next to
> the hexagonal ones in my experiments. And all were oily when touched. It is
> very likely that my "detergent" crystals were in reality detergent-protein
>
Dear,
When (trying) to refine a DNA-structure (resolution 2.5) using
Refmac_5.5.0072 (CCP4 6.1.1), some of the H-bonds between Watson-
Crick bases are becoming too large.
Reducing the Matrix weighting term to tighten the geometry, doesn't
effect these H-bond distances much.
Reducing the "
Hi Kristof
AFAIK there's no attractive H-bond term in Refmac, only a repulsive one,
and even that is probably between the N/O atoms not between the H atoms
themselves, which only 'ride' on their parent atoms (again this is to my
knowledge). This is all related to the fact that H atoms don't scatt
Dear all
Can anyone suggest examples where the same peptide is bound to the same
receptor site in two or more different (very different) conformations
and this binding has been resolved crystallographically as well as using
ITC. In the extreme cases this could be two different proteins as well
ref 10289
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The School of Crystallography/Institute of Structural and Molecular
Biology is seeking a Post-doctoral Research Assistant to carry out
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Hi Kristof -
I think that you are overlooking at the fact that you do not know what
the hydrogen bond lengths are between the base pairs.
You should make sure that the covalent geometry is correct (tighten or
loosen the matrix as you do!) and then when you
have a well refined structure, analy
Dear All,
The Lieberman Lab, located in the School of Chemistry & Biochemistry
at the Georgia Institute of Technology (Atlanta, GA) invites highly
motivated individuals interested in the field of protein misfolding
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Hi all
I did some HA searching and found some sites in C222 that seem to be
NCS related. Any ideas on how to determine whether or not the space
group is really C2221 from these sites?
Thanks
FR
-
Francis Reyes M.Sc.
215 UCB
University of Colorad
I would expect that if you found HA sites in C222 then they must obey
C222 symmetry?
Why not just repeat the search in C2221 and see if you get a better answer?
Going back a stage:
Arent you able to guess whether there is a screw axis from your absences
along 00l?
Pointless gives a good ana
Hello All-
I figured I would post this here since there are some protein
biochemists who frequent the site, and many of the crystallographers may
know of protein biochemists who might be looking for a job. Please pass
this along to any potentially interested parties.
Thanks!
Steve
P.S. Please
Dear Sir or Madam,
The ICP-ES results indicated that 1 molar my protein purified from E.coli
Origami(DE3) contained about a half molar Zinc and nearly a quarter
molar Iron (whether II or III was not available). The protein carried a MBP
tag on the N-terminal and the situation was similar with or w
Hi Xuan,
I guess your protein is not an E.coli protein. There are several
examples that eukaryotic Zn-proteins expressed in E.coli contain Fe
instead of Zn. I am sceptic whether IMAC with different metal ions will
give the solution of the problem. If you really want to get information
on the m
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