Re: [ccp4bb] tips on crystallizing a Protein-DNA complex

2009-07-25 Thread peter hudson
Hello all This a follow up of the mail "tips on crystallisation of protein-DNA complex." I would be interested to know about the purity of DNA used for the DNA-complex crystallisation. What does mean by initial screening with the unpurified DNA? Is this mean to start with the desalting DNA rather

[ccp4bb] Density modification

2009-07-25 Thread kumar
Dear CCP4¹ers, I¹m trying to run DM for after Refmac on an MR solution but it crashes. I do have the FOM¹s listed in my log file. I get a similar error when I use the mtz file from Phaser¹s SAD + MR. I¹d appreciate any help. Thanks! Kumar After Refmac ŠŠ.. HISTLIB opened: Number of hi