It is possible and should definitely be tried.
SHARP works very well for MIRAS phasing in our experience.
-Bjørn
Arnon Lavie wrote:
Hi,
we collected SAD data sets to 3.0 A on our protein, once as a Se-Met
protein and once soaked with a mercury compound. Data statistics
indicate an anomalous
Dear Eike,
depending on what your structure is - you can try interface search in
PISA (google on "ebi pisa"). Select the most "representative" interface
in your complex (the one that makes the non-standard arrangement) and
see whether there are similar interfaces in the PDB. It shouldn't be
a too
Dear all:
I have a problem in locating heavy atom in P1 space group.My
protein is 169 residue.There are 2 molecular per asymmetry unit
according Matthews coefficient.I have soaked my protein in Hg
solution.I collected the data at 1A wavelength and the anomalous d" is
about 3 at low resoluti
Dear crystallographers,
I want to post my intention of sale of our Rigaku MicroMax-007 HFM X-ray
generator. The generator was originally purchased by Scripps FL in 2007 but
was replace with a new generator by an insurance company shortly after that.
So we are left with two generators and selling th
Dear ccp4bb,
I've recently run into a problem refining a crystal structure that I indexed
and performed a molecular replacement on as C2. The unit cell dimensions are
a=96, b=95.8, c=58.1 and beta=127; Rmrg=.195. The data go down to a resolution
of 2.8 angstroms. The data also index in I222,
Hi Jeffrey
Re-indexing this in the conventional setting (i.e. beta nearest to 90 -
for reasons no-one has yet been able to explain to me the standard d.p.
programs do not make this choice automatically!) gives you space group
I2 with cell:
a=58.1 b=95.8 c=76.67 beta=90.24
With beta now so clo
There is a very useful program written by Phil Evans called othercell
which gives alternate indexing
( The same functionality is in pointless)
In this case it suggests:
Alternative indexing schemes which lead to identical or similar
cells are grouped on continuation lines if they are symmetry-r
If you have just one heavy atom in space group P1 you can simply place it
at the origin (0,0,0). Phasing with SHELXC and SHELXE should then work
fine if you have SAD, MAD or SIRAS data but if you only use SIR your map
will be a double image that it will not be possible to resolve. Are you
sure
Principal Scientist - Protein Crystallography
Australian Synchrotron
The Australian Synchrotron, a major new national facility, provides researchers
from Australia, NZ and overseas with a powerful new tool for scientific and
industrial research. The facility has applications in fields as diverse
POST DOCTORAL RESEARCH ASSOCIATE
The National Institute of General Medical Sciences East Coast Structural
Biology Research Facility, X6A beam line at the NSLS, Brookhaven National
Laboratory has an immediate opening for Postdoctoral Research Associate
position. Funded by the National Institute
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