Re: [ccp4bb] Cross-crystal density averaging and/or refinement

Have you tried DMMULTI? It can do a brilliant job.. Eleanor Ethan Merritt wrote: Hi all, I have an interesting problem case at the moment. We have crystallized the same 2-domain protein in two different, i.e. non-isomorphous, crystal forms. There is a decent homologous structure for one doma

Re: [ccp4bb] Cross-crystal density averaging and/or refinement

Ethan Merritt schrieb: Hi all, I have an interesting problem case at the moment. We have crystallized the same 2-domain protein in two different, i.e. non-isomorphous, crystal forms. There is a decent homologous structure for one domain (about 40% of the total), but no known homologous struct

Re: [ccp4bb] ccp4mg question

Ambrose Cole wrote: I've found out how to do it on ccp4mg but I was working with the new QtMG, still can't find where to do it there though. Ambrose Dear Ambrose, There should be an option "Colour browser.." on the colour menu. Click on the "Create colours" tab to add whatever colour you lik

[ccp4bb] Permanent industrial position for a PhD crystallographer in Munich, southern Germany

Dear all, Are you tired of protein expression and purification? Want to concentrate on Crystallisation and Crystallography only? Are you interested in working in a very lively and young company with about 45 colleagues of talented scientists and technical staff in a pleasant atmosphere? Wou

Re: [ccp4bb] Permanent industrial position for a PhD crystallographer in Munich, southern Germany

Only problem: it's in Bavaria... and we belive that you will need an excellent command of the German language both written and spoken to work here efficiently and live here happily. Dear das Doktor von Hannes! Ich das Jobb very interessiert ist worden worden geworden. Die Bayern placement nic

Re: [ccp4bb] Permanent industrial position for a PhD crystallographer in Munich, southern Germany

Gerard, I think you have no time left to enjoy the beer and discuss in the native language with the bavarians With about five structures a day, you will have your hands full on doing a good job if you work to fast and leave it up to automation, you are bound to miss the bound inhibitors.

[ccp4bb] unknown density

Dear all, i am working on a structure of M.tb, solved at 2.18 A. In my structure there is a density near to the active site and we modelled it as a malonate (C3 O4 H2) molecule acorrding to the density, which was not used in any of our buffer except we used 10 mM DTT, 1.6 M Ammonium sulphate, 100 m

Re: [ccp4bb] unknown density

Theoretically, citric acid can decompose into beta-ketoglutaric acid, and the latter may decarboxylate into malonic acid... Not sure if this happens in aqueous solutions, but if your citrate had malonate contamination and your enzyme prefers malonate to anything else - that's where it may have com

[ccp4bb] X-ray equipment available

To all colleagues at CCP4BB: We have the following X-ray diffraction equipment to give away: I. Siemens X-1000 Multiwire Area Detector. It has been manufactured by Siemens Analytical X-ray Instruments Inc. in Madison, Wisconsin, USA. Equipment available includes multiwire area detector X-10

[ccp4bb] Rice vs Sim vs ACNSFD

Dear All, I am unsure about the likelihood function nomenclature. I have seen the Sim function called the Rice function, and aIso have read the acentric conditional normalized structure factor distribution (ACNSFD) called the Rice function (the centric conditional normalized structure fa