[ccp4bb] Postdoc Crystallographer Position at Swiss Light Source

2009-01-15 Thread Meitian Wang
Postdoctoral Fellow Protein Crystallography with PILATUS detector Your tasks After one and a half years of successful operation of the first two- dimensional hybrid pixel array detector (PILATUS 6M) at the beamline X06SA, some of its advantages and outstanding capabilities for macromolecul

Re: [ccp4bb] Ramachandran plot, in a text list

2009-01-15 Thread Eleanor Dodson
John Pak wrote: Hi, sorry if this was posted earlier. How can I calculate a Ramachandran plot, but output the information to a text file list? i.e. something like Ala51, phi=X, psi=Y. I can't seem to find this information in the ccp4 SFCheck/Procheck log file. I thought PROCHECK listed

Re: [ccp4bb] Ramachandran plot, in a text list

2009-01-15 Thread Martyn Winn
However, procheck ramachandran is deprecated these days as it is based on older data than say molprobity. Obviously that won't matter just for a list of phi/psi angles, but might affect selections e.g. what it thinks are in a helix (cf Eleanor's message) m On Wed, 2009-01-14 at 14:39 -0500, Edwar

Re: [ccp4bb] Ramachandran plot, in a text list

2009-01-15 Thread MATSUURA Takanori
Hi, How about STAN server at Uppsala? http://xray.bmc.uu.se/cgi-bin/gerard/rama_server.pl I suppose you can find what you are looking for at "MOLEMAN2 main-chain analysis" in the output webpage. Takanori From: John Pak Subject: [ccp4bb] Ramachandran plot, in a text list Date: Wed, 14 Jan

Re: [ccp4bb] Ligand binding in multiple conformation

2009-01-15 Thread Chandra Verma
An extension of this topic: does anyone know of examples where for complexes between the two proteins A and B, protein A has two different conformations at its interface? thanks chandra -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Aaron O

Re: [ccp4bb] Ramachandran plot, in a text list

2009-01-15 Thread John Pak
Thanks for the replies everyone. I ended up using CNS_SOLVE 1.1 to generate the Ramachandran list. I don't seem to have access to the .rin file you mentioned. The only output I get from Procheck are the log file and the various postscript files. No big deal, perhaps it's because I'm runn

[ccp4bb] Postdoctoral positions available, Xiamen, China

2009-01-15 Thread aidong
A structural biology lab has now several openings. This lab conducts two aspects of research using X-ray crystallography and other biochemical approaches. One is to understand how a limited number of transcriptional factors regulate a large amount of genes. Our model is that two or more

[ccp4bb] Crystallography plates, hanging drop but templated sealing film.

2009-01-15 Thread Francis E Reyes
Does such a thing exist? A 24-well microplate configuration where in substitution of glass cover slips, you have a roll of tape templated such that there are circular areas where you can add your protein where there is no adhesive, but there is adhesive everywhere else? This may be a nightm

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

2009-01-15 Thread Diana Tomchick
Are you saying that you'd like to re-use the plate with the original screening solutions, or that you plan to clean the plates, then dispense fresh screening solutions? If you would like to re-use the original screening solutions, beware...after many weeks, they will not be the same concent

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

2009-01-15 Thread Patrick Loll
This sounds very similar to a nifty little device the folks in Buffalo came up with: J. Appl. Cryst. (1992). 25, 324-325[ doi:10.1107/S0021889891011354 ] HANGMAN: a macromolecular hanging-drop vapor-diffusion technique J. R. Luft and G. T. DeTitta On 15 Jan 2009, at 4:34 PM, Francis E Rey

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

2009-01-15 Thread Leonard Thomas
Why not just use a 96 well plate. Should be able to do this by hand with a large enough drop. You can get them from either TTP Labtech or Grace Biosystems, I think that is the name. Cheers, Len Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory Manager University of Oklahoma D