Postdoctoral Fellow
Protein Crystallography with PILATUS detector
Your tasks
After one and a half years of successful operation of the first two-
dimensional hybrid pixel array detector (PILATUS 6M) at the beamline
X06SA, some of its advantages and outstanding capabilities for
macromolecul
John Pak wrote:
Hi, sorry if this was posted earlier. How can I calculate a
Ramachandran plot, but output the information to a text file list?
i.e. something like Ala51, phi=X, psi=Y.
I can't seem to find this information in the ccp4 SFCheck/Procheck log
file.
I thought PROCHECK listed
However, procheck ramachandran is deprecated these days as it is based
on older data than say molprobity. Obviously that won't matter just for
a list of phi/psi angles, but might affect selections e.g. what it
thinks are in a helix (cf Eleanor's message)
m
On Wed, 2009-01-14 at 14:39 -0500, Edwar
Hi,
How about STAN server at Uppsala?
http://xray.bmc.uu.se/cgi-bin/gerard/rama_server.pl
I suppose you can find what you are looking for at "MOLEMAN2
main-chain analysis" in the output webpage.
Takanori
From: John Pak
Subject: [ccp4bb] Ramachandran plot, in a text list
Date: Wed, 14 Jan
An extension of this topic: does anyone know of examples where for
complexes between the two proteins A and B, protein A has two different
conformations at its interface?
thanks
chandra
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Aaron O
Thanks for the replies everyone. I ended up using CNS_SOLVE 1.1 to
generate the Ramachandran list.
I don't seem to have access to the .rin file you mentioned. The only
output I get from Procheck are the log file and the various postscript
files. No big deal, perhaps it's because I'm runn
A structural biology lab has now several openings. This lab conducts
two aspects of research using X-ray crystallography and other
biochemical approaches. One is to understand how a limited number of
transcriptional factors regulate a large amount of genes. Our model
is that two or more
Does such a thing exist? A 24-well microplate configuration where in
substitution of glass cover slips, you have a roll of tape templated
such that there are circular areas where you can add your protein
where there is no adhesive, but there is adhesive everywhere else?
This may be a nightm
Are you saying that you'd like to re-use the plate with the original
screening solutions, or that you plan to clean the plates, then
dispense fresh screening solutions?
If you would like to re-use the original screening solutions,
beware...after many weeks, they will not be the same concent
This sounds very similar to a nifty little device the folks in
Buffalo came up with:
J. Appl. Cryst. (1992). 25, 324-325[ doi:10.1107/S0021889891011354 ]
HANGMAN: a macromolecular hanging-drop vapor-diffusion technique
J. R. Luft and G. T. DeTitta
On 15 Jan 2009, at 4:34 PM, Francis E Rey
Why not just use a 96 well plate. Should be able to do this by hand
with a large enough drop. You can get them from either TTP Labtech or
Grace Biosystems, I think that is the name.
Cheers,
Len
Leonard Thomas Ph. D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
D
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