Re: [ccp4bb] Potential N-terminal cyclization cont.

2008-10-11 Thread Prof. Dr. Arne Skerra
Dear Gerwald, dear Artem, As, in the absence of quantum mechanical calculations, we can only speculate about the contribution of mesomeric states it seems to me from the data presented that the left formula (i.e. without the mesomeric effect) may match the structural situation, in particular

Re: [ccp4bb] Substrate(water insoluble) Soaking

2008-10-11 Thread George Kontopidis
Take one crystal and put it in mother liquor. Increase gradually (in two separately experiments) your ethanol and DMSO concentration (in my experience it could go up to 10%v/v after 1 week for some crystals) in order to find out the concentration that your crystal can stand without dissolve or crac