Dear friends,
I have two problems with my proteins.
For protein A, it was purified using the Nickel-affinity purification
method. If the sample was placed at 4 degrees centigrade for 24 hrs, it
didn't change
obviously. But if it was concentrated by centrifuge at 4 degrees for further
gel-f
My experience is the same as Frank's. As I compete a structure for which
I am using TLS restraints, I often refine in both phenix and refmac and
sometimes go back and forth. I am confused as to why refmac keeps the
ANSIOU cards and doesn't keep its output consistent. It would be nice if
the
Hi,
Just a newbie question:
Could someone explain what might have gone wrong in this case? I guess
the structure factors should not change anyway! I am a bit confused
because I have used solve resolve several times for experimental
phasing, never had such a problem, on the other hand, have not he
Hi Partha,
It sounds to me as though the amplitudes that were given to resolve as FP
may have been calculated ones. The FP and SIGFP written out by resolve
are normally the same as those that are input, and so you can use them in
refinement in most circumstances. You are always safest to do as G
