Dear all,
Does anybody have some good experiences or methods to adjust the
outlier dihedral angles? I have a model most of
which was builded manually, so though the Rfree is
acceptable--0.29--manydihedral angles are not right. it is difficult
to me
to correct them. If there is any software to
Li Yang,
I usually use three ways to solve that:
1 CNS anneal
2 coot edit backbone torsions
and the last redo manual build as Paul's suggestions for low resolution
data in COOT FAQ if you using coot and the resolution of your data is low
Good Luck!
liu
yang li wrote:
Dear all,
Does any
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Hi all
sorry, for offtopic query...
I am trying to purify my protein by Ni-NTA affinity chromatography. After
sonication as i centrifuge bacterial lysate, soon after 10 min whole lysates
get precipitated during loading on the column and some time it remain
soluble too. if i get purified through t
If you really tried all sorts of buffers (did you go to extreme pH-values,
very high salt concentrations, tried various additives (beta-ME,
glycerol,...), different salts)
you can still
- try another expression system (insect cells, mammalian)
- see if any modifications might be useful
- try res
